X-Gal BioChemica

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Name X-Gal BioChemica
Product Type Chemical reagent
Supplier BioChemica
Form Powder
Color White
Purity ≥ 98%
Solubility Soluble in DMSO, DMF, and water
Storage Condition Store at -20°C
Applications Used in molecular biology for blue/white screening of recombinant bacterial colonies
Cas Number 7240-90-6
Molecular Formula C14H15BrClNO6
Molecular Weight 408.63 g/mol
FAQ
What is X-Gal BioChemica and how is it used in laboratory research?

X-Gal BioChemica is a chromogenic substrate commonly used in molecular biology and biochemistry research. It is often utilized in conjunction with β-galactosidase to identify and analyze gene expressions in bacterial and mammalian cells. X-Gal BioChemica is a colorless substrate that turns blue when cleaved by β-galactosidase, allowing researchers to visually detect and quantify gene expression levels.

How does X-Gal BioChemica differ from other chromogenic substrates on the market?

X-Gal BioChemica is known for its high purity and reliability, making it a preferred choice for researchers conducting gene expression studies. Unlike other chromogenic substrates, X-Gal BioChemica offers consistent and reproducible results, ensuring accurate and dependable data in laboratory experiments.

What are the advantages of using X-Gal BioChemica in gene expression analysis?

One of the main advantages of using X-Gal BioChemica is its sensitivity in detecting gene expression levels. It allows researchers to visually assess gene activity, making it a valuable tool in studying gene regulation and expression patterns. Additionally, X-Gal BioChemica is easy to use and cost-effective, making it a popular choice for many research laboratories.

How can X-Gal BioChemica be incorporated into experimental protocols?

X-Gal BioChemica can be easily added to agar plates or liquid media for bacterial or mammalian cell culture experiments. Researchers can prepare X-Gal solutions at various concentrations depending on the desired sensitivity and signal intensity. After incubation with X-Gal BioChemica, cells expressing the target gene will exhibit a blue color, allowing for quick and efficient analysis of gene expression levels.

In what types of research applications is X-Gal BioChemica commonly used?

X-Gal BioChemica is widely used in molecular biology and biochemistry research, particularly in studies involving gene expression, recombinant DNA technology, and protein analysis. It is frequently employed in screening assays, reporter gene analysis, and transfection experiments to assess gene activity and regulatory mechanisms. Overall, X-Gal BioChemica is a versatile tool that can benefit researchers across various disciplines.
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