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| Product Name | 2-Propanol for HPLC |
| CAS Number | 67-63-0 |
| Molecular Formula | C3H8O |
| Molecular Weight | 60.10 g/mol |
| Purity | ≥ 99.9% |
| Appearance | Colorless liquid |
| Boiling Point | 82.5 °C |
| Density | 0.785 g/mL |
| Solubility | Miscible in water |
| Storage | Store in a cool, dry, well-ventilated area away from incompatible substances |
| Handling | Avoid contact with eyes, skin, and clothing |
| Applications | Used as a solvent in high-performance liquid chromatography (HPLC) |
| Synonyms | Isopropanol, IPA, Propan-2-ol |
FAQ
What is `2-Propanol` and how is it used in HPLC?
`2-Propanol`, also known as isopropanol or isopropyl alcohol, is a common solvent used in various analytical techniques, including High Performance Liquid Chromatography (HPLC). In HPLC, `2-Propanol` is often used as a mobile phase solvent due to its ability to dissolve a wide range of organic compounds. It is particularly useful for separating and analyzing compounds that are non-polar or have limited solubility in water.
How does `2-Propanol` improve HPLC performance?
`2-Propanol` offers several advantages when used in HPLC applications. It has a low viscosity, which helps to improve flow rates and efficiency in the chromatographic process. Additionally, `2-Propanol` evaporates quickly, which aids in the detection and quantification of analytes. Its compatibility with a wide range of detectors makes it a versatile solvent choice for various HPLC methods.
What are some key considerations when using `2-Propanol` in HPLC?
When using `2-Propanol` in HPLC, it is important to consider its compatibility with stationary phases and columns. Some stationary phases may be sensitive to `2-Propanol`, leading to reduced column lifetime or performance. It is also crucial to ensure proper solvent purity and quality to prevent contamination of samples and interference with analyte detection. Regular maintenance and monitoring of the HPLC system are essential to prevent issues related to solvent interactions and column degradation.
Can `2-Propanol` be used in combination with other solvents in HPLC?
Yes, `2-Propanol` can be used in combination with other solvents to optimize the separation of analytes in HPLC. Common solvent mixtures include `2-Propanol` with water or acetonitrile, depending on the nature of the compounds being analyzed. The choice of solvent composition and ratios can significantly impact the selectivity and resolution of chromatographic peaks in HPLC. Experimentation with different solvent systems is often necessary to achieve the desired separation performance.
What are the storage and handling considerations for `2-Propanol` in HPLC applications?
Proper storage and handling of `2-Propanol` are essential to maintain its quality and performance in HPLC applications. It should be stored in a cool, dry place away from direct sunlight and sources of heat to prevent evaporation and degradation. Handling `2-Propanol` in a well-ventilated area and using appropriate personal protective equipment is recommended to ensure safety. Regular inspection of solvent containers for leaks or contamination is crucial to prevent cross-contamination of samples and maintain the integrity of the HPLC system.
`2-Propanol`, also known as isopropanol or isopropyl alcohol, is a common solvent used in various analytical techniques, including High Performance Liquid Chromatography (HPLC). In HPLC, `2-Propanol` is often used as a mobile phase solvent due to its ability to dissolve a wide range of organic compounds. It is particularly useful for separating and analyzing compounds that are non-polar or have limited solubility in water.
How does `2-Propanol` improve HPLC performance?
`2-Propanol` offers several advantages when used in HPLC applications. It has a low viscosity, which helps to improve flow rates and efficiency in the chromatographic process. Additionally, `2-Propanol` evaporates quickly, which aids in the detection and quantification of analytes. Its compatibility with a wide range of detectors makes it a versatile solvent choice for various HPLC methods.
What are some key considerations when using `2-Propanol` in HPLC?
When using `2-Propanol` in HPLC, it is important to consider its compatibility with stationary phases and columns. Some stationary phases may be sensitive to `2-Propanol`, leading to reduced column lifetime or performance. It is also crucial to ensure proper solvent purity and quality to prevent contamination of samples and interference with analyte detection. Regular maintenance and monitoring of the HPLC system are essential to prevent issues related to solvent interactions and column degradation.
Can `2-Propanol` be used in combination with other solvents in HPLC?
Yes, `2-Propanol` can be used in combination with other solvents to optimize the separation of analytes in HPLC. Common solvent mixtures include `2-Propanol` with water or acetonitrile, depending on the nature of the compounds being analyzed. The choice of solvent composition and ratios can significantly impact the selectivity and resolution of chromatographic peaks in HPLC. Experimentation with different solvent systems is often necessary to achieve the desired separation performance.
What are the storage and handling considerations for `2-Propanol` in HPLC applications?
Proper storage and handling of `2-Propanol` are essential to maintain its quality and performance in HPLC applications. It should be stored in a cool, dry place away from direct sunlight and sources of heat to prevent evaporation and degradation. Handling `2-Propanol` in a well-ventilated area and using appropriate personal protective equipment is recommended to ensure safety. Regular inspection of solvent containers for leaks or contamination is crucial to prevent cross-contamination of samples and maintain the integrity of the HPLC system.
