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| Name | 2-Propanol |
| Cas Number | 67-63-0 |
| Molecular Formula | C3H8O |
| Molecular Weight | 60.10 g/mol |
| Appearance | Colorless liquid |
| Boiling Point | 82.5°C |
| Melting Point | -89°C |
| Density | 0.785 g/cm3 |
| Solubility | Miscible in water |
| Purity | ≥99.5% |
| Storage Conditions | Store at room temperature in a dry place |
| Usage | Commonly used as a solvent in molecular biology applications |
| Hazards | Flammable liquid, eye irritant |
| Derivation | Obtained from the hydration of propylene |
| Odor | Characteristic alcohol odor |
| Flash Point | 11.7°C |
FAQ
What is 2-Propanol and how is it used in molecular biology?
2-Propanol is a common chemical compound also known as isopropyl alcohol. In molecular biology, it is used for a variety of purposes such as DNA extraction, DNA precipitation, RNA extraction, protein precipitation, and as a solvent for PCR reactions.
How is 2-Propanol different from other types of alcohols commonly used in molecular biology?
2-Propanol is preferred over other types of alcohols in molecular biology due to its lower cost, lower toxicity, and higher purity. It is also less likely to cause denaturation of biomolecules compared to other alcohols like ethanol.
Can 2-Propanol be used for RNA extraction in molecular biology applications?
Yes, 2-Propanol can be used for RNA extraction in molecular biology applications. It is commonly used for RNA precipitation to isolate RNA from a solution containing DNA, proteins, and other contaminants.
Is 2-Propanol suitable for use in PCR reactions in molecular biology?
Yes, 2-Propanol is suitable for use in PCR reactions in molecular biology. It is commonly used as a solvent in PCR reactions to dissolve and dilute primers, nucleotides, DNA template, and polymerase enzymes.
Are there any safety considerations to keep in mind when working with 2-Propanol in molecular biology?
Yes, there are several safety considerations to keep in mind when working with 2-Propanol in molecular biology. It is flammable, so it should be stored and handled away from open flames and heat sources. It is also an irritant to the skin, eyes, and respiratory system, so proper personal protective equipment such as gloves, goggles, and lab coats should be worn when working with it. Additionally, it should be used in a well-ventilated area to prevent inhalation of its fumes.
2-Propanol is a common chemical compound also known as isopropyl alcohol. In molecular biology, it is used for a variety of purposes such as DNA extraction, DNA precipitation, RNA extraction, protein precipitation, and as a solvent for PCR reactions.
How is 2-Propanol different from other types of alcohols commonly used in molecular biology?
2-Propanol is preferred over other types of alcohols in molecular biology due to its lower cost, lower toxicity, and higher purity. It is also less likely to cause denaturation of biomolecules compared to other alcohols like ethanol.
Can 2-Propanol be used for RNA extraction in molecular biology applications?
Yes, 2-Propanol can be used for RNA extraction in molecular biology applications. It is commonly used for RNA precipitation to isolate RNA from a solution containing DNA, proteins, and other contaminants.
Is 2-Propanol suitable for use in PCR reactions in molecular biology?
Yes, 2-Propanol is suitable for use in PCR reactions in molecular biology. It is commonly used as a solvent in PCR reactions to dissolve and dilute primers, nucleotides, DNA template, and polymerase enzymes.
Are there any safety considerations to keep in mind when working with 2-Propanol in molecular biology?
Yes, there are several safety considerations to keep in mind when working with 2-Propanol in molecular biology. It is flammable, so it should be stored and handled away from open flames and heat sources. It is also an irritant to the skin, eyes, and respiratory system, so proper personal protective equipment such as gloves, goggles, and lab coats should be worn when working with it. Additionally, it should be used in a well-ventilated area to prevent inhalation of its fumes.
