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| Name | Ethanol absolute |
| Purity | 99.5% |
| Grade | Molecular biology grade |
| Appearance | Clear colorless liquid |
| Solubility | Soluble in water |
| Storage Condition | Store at room temperature |
| Cas Number | 64-17-5 |
| Molecular Formula | C2H6O |
| Molecular Weight | 46.07 g/mol |
| Boiling Point | 78.37°C |
| Flash Point | 13°C |
FAQ
What is ethanol absolute and why is it used in molecular biology?
Ethanol absolute, also known as absolute ethanol, is a pure form of ethanol that contains little to no water. In molecular biology, it is commonly used as a solvent for nucleic acids, proteins, and other biomolecules. Its ability to denature proteins and disrupt hydrogen bonding makes it an essential reagent in various laboratory procedures.
How is ethanol absolute different from other forms of ethanol?
Ethanol absolute differs from other forms of ethanol, such as denatured ethanol or ethanol solutions, in its purity level. Absolute ethanol is at least 99.5% pure, whereas other forms may contain additives or impurities that make them unsuitable for certain molecular biology applications. The lack of water in ethanol absolute also distinguishes it from ethanol solutions, which have varying concentrations of water.
What are some common uses of ethanol absolute in molecular biology?
Ethanol absolute is used in molecular biology for a variety of purposes, including DNA extraction, RNA precipitation, protein precipitation, and cell fixation. It is commonly used in techniques such as DNA and RNA isolation, PCR cleanup, and protein purification. Its ability to precipitate nucleic acids and proteins makes it a versatile reagent in molecular biology research.
How should ethanol absolute be stored and handled in the laboratory?
Ethanol absolute should be stored in a cool, dry place away from heat sources and direct sunlight. It should be kept in a tightly sealed container to prevent evaporation and contamination. Proper labeling with hazard warnings and handling instructions is crucial to ensure the safety of laboratory personnel. When working with ethanol absolute, it is important to wear appropriate personal protective equipment, such as gloves and goggles, to avoid skin contact and inhalation of fumes.
Are there any safety precautions to consider when using ethanol absolute?
Yes, there are several safety precautions to consider when using ethanol absolute in the laboratory. Ethanol absolute is highly flammable and should be kept away from open flames and sources of ignition. It can also be harmful if ingested or inhaled, so it should be used in a well-ventilated area and handled with care. Avoid skin contact with ethanol absolute, as it can cause irritation and dryness. In case of accidental exposure, rinse the affected area with water and seek medical attention if necessary.
Ethanol absolute, also known as absolute ethanol, is a pure form of ethanol that contains little to no water. In molecular biology, it is commonly used as a solvent for nucleic acids, proteins, and other biomolecules. Its ability to denature proteins and disrupt hydrogen bonding makes it an essential reagent in various laboratory procedures.
How is ethanol absolute different from other forms of ethanol?
Ethanol absolute differs from other forms of ethanol, such as denatured ethanol or ethanol solutions, in its purity level. Absolute ethanol is at least 99.5% pure, whereas other forms may contain additives or impurities that make them unsuitable for certain molecular biology applications. The lack of water in ethanol absolute also distinguishes it from ethanol solutions, which have varying concentrations of water.
What are some common uses of ethanol absolute in molecular biology?
Ethanol absolute is used in molecular biology for a variety of purposes, including DNA extraction, RNA precipitation, protein precipitation, and cell fixation. It is commonly used in techniques such as DNA and RNA isolation, PCR cleanup, and protein purification. Its ability to precipitate nucleic acids and proteins makes it a versatile reagent in molecular biology research.
How should ethanol absolute be stored and handled in the laboratory?
Ethanol absolute should be stored in a cool, dry place away from heat sources and direct sunlight. It should be kept in a tightly sealed container to prevent evaporation and contamination. Proper labeling with hazard warnings and handling instructions is crucial to ensure the safety of laboratory personnel. When working with ethanol absolute, it is important to wear appropriate personal protective equipment, such as gloves and goggles, to avoid skin contact and inhalation of fumes.
Are there any safety precautions to consider when using ethanol absolute?
Yes, there are several safety precautions to consider when using ethanol absolute in the laboratory. Ethanol absolute is highly flammable and should be kept away from open flames and sources of ignition. It can also be harmful if ingested or inhaled, so it should be used in a well-ventilated area and handled with care. Avoid skin contact with ethanol absolute, as it can cause irritation and dryness. In case of accidental exposure, rinse the affected area with water and seek medical attention if necessary.
