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Name | Ethanol absolute for molecular biology (untaxed) |
Type | Chemical reagent |
Purity | Absolute |
Grade | Molecular biology grade |
Application | Molecular biology research |
Solubility | Soluble in water |
Storage | Store at room temperature |
Appearance | Clear, colorless liquid |
Formula | C2H6O |
CAS Number | 64-17-5 |
Molar Mass | 46.07 g/mol |
FAQ
What is ethanol absolute and how is it used in molecular biology?
Ethanol absolute is a pure form of ethanol that contains no water. In molecular biology, it is commonly used as a solvent for molecular biology reagents, such as DNA or RNA precipitation and extraction.
Why is ethanol absolute preferred over other forms of ethanol in molecular biology applications?
Ethanol absolute is preferred over other forms of ethanol because it contains no water, making it more effective at precipitating nucleic acids and other biomolecules. Water can interfere with these processes by diluting the solution and reducing the efficiency of the precipitation.
What are some common applications of ethanol absolute in molecular biology?
Ethanol absolute is commonly used in molecular biology for DNA and RNA precipitation, protein precipitation, DNA and RNA extraction, and as a solvent for nucleic acid purification kits. It is also used for DNA and RNA isolation from agarose gels and as a solvent for other chemical reagents.
How should ethanol absolute be stored and handled in the laboratory?
Ethanol absolute should be stored in a cool, dry place away from direct sunlight and sources of heat. It should be kept in a tightly sealed container to prevent evaporation and contamination. When handling ethanol absolute, it is important to wear appropriate personal protective equipment, such as gloves and goggles, to protect against skin and eye irritation.
Are there any safety considerations when working with ethanol absolute in the laboratory?
Ethanol absolute is highly flammable and should be handled with caution in the laboratory. It should only be used in well-ventilated areas, away from open flames or spark-producing equipment. It should also be kept away from oxidizing materials, as it can react violently in the presence of strong oxidizers. Additionally, ethanol absolute should not be ingested or inhaled, as it can cause serious health effects.
Ethanol absolute is a pure form of ethanol that contains no water. In molecular biology, it is commonly used as a solvent for molecular biology reagents, such as DNA or RNA precipitation and extraction.
Why is ethanol absolute preferred over other forms of ethanol in molecular biology applications?
Ethanol absolute is preferred over other forms of ethanol because it contains no water, making it more effective at precipitating nucleic acids and other biomolecules. Water can interfere with these processes by diluting the solution and reducing the efficiency of the precipitation.
What are some common applications of ethanol absolute in molecular biology?
Ethanol absolute is commonly used in molecular biology for DNA and RNA precipitation, protein precipitation, DNA and RNA extraction, and as a solvent for nucleic acid purification kits. It is also used for DNA and RNA isolation from agarose gels and as a solvent for other chemical reagents.
How should ethanol absolute be stored and handled in the laboratory?
Ethanol absolute should be stored in a cool, dry place away from direct sunlight and sources of heat. It should be kept in a tightly sealed container to prevent evaporation and contamination. When handling ethanol absolute, it is important to wear appropriate personal protective equipment, such as gloves and goggles, to protect against skin and eye irritation.
Are there any safety considerations when working with ethanol absolute in the laboratory?
Ethanol absolute is highly flammable and should be handled with caution in the laboratory. It should only be used in well-ventilated areas, away from open flames or spark-producing equipment. It should also be kept away from oxidizing materials, as it can react violently in the presence of strong oxidizers. Additionally, ethanol absolute should not be ingested or inhaled, as it can cause serious health effects.