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| Name | Digitonin (Reag. USP) |
| Brand | Reag. USP |
| Type | Saponin |
| Source | Plant-derived |
| Form | Powder |
| Purity | ≥98% |
| Solubility | Soluble in methanol, ethanol, chloroform |
| Storage | Store at room temperature |
| Applications | Biochemical research, cell biology |
| CAS Number | 11024-24-1 |
| Molecular Weight | 1227.44 g/mol |
FAQ
What is Digitonin (Reag. USP) and how does it work?
Digitonin (Reag. USP) is a natural saponin derived from the seeds of the plant Digitalis purpurea. It is widely used in biochemistry and cell biology research as a nonionic detergent that selectively permeabilizes the plasma membrane of cells. Digitonin forms complexes with cholesterol in the cell membrane, allowing for the release of cytoplasmic components while preserving the integrity of intracellular organelles.
What are the benefits of using Digitonin (Reag. USP) in research?
Digitonin (Reag. USP) offers several advantages in research applications. It is a gentle and effective detergent that can be used for the solubilization and isolation of membrane proteins without denaturing them. Digitonin also has the ability to selectively permeabilize the plasma membrane, making it a useful tool for studying the interactions between proteins within cells. Additionally, Digitonin is compatible with a wide range of biochemical assays, making it a versatile reagent for various research applications.
How is Digitonin (Reag. USP) typically used in experiments?
Digitonin (Reag. USP) is commonly used in experiments to permeabilize cells for the study of intracellular proteins and processes. Researchers typically prepare a digitonin solution at a specific concentration and treat cells with the detergent for a defined period of time. Digitonin selectively permeabilizes the plasma membrane, allowing researchers to investigate the localization, interaction, and function of intracellular proteins. Following treatment with digitonin, cells can be further analyzed using techniques such as immunofluorescence, western blotting, or enzyme activity assays.
Are there any specific considerations when using Digitonin (Reag. USP) in experiments?
When using Digitonin (Reag. USP) in experiments, researchers should consider several factors to ensure optimal results. It is important to determine the appropriate concentration of digitonin for permeabilizing cells based on the specific experimental requirements. Additionally, researchers should optimize the treatment time and conditions to minimize nonspecific effects on cellular components. It is also recommended to validate the integrity of intracellular organelles following digitonin treatment to ensure the preservation of cellular structures. Overall, careful experimental design and optimization are essential for successful applications of Digitonin in research.
In conclusion, Digitonin (Reag. USP) is a valuable reagent for studying membrane proteins and intracellular processes in research. Its gentle and selective permeabilization properties make it a versatile tool for a wide range of applications in biochemistry and cell biology. By understanding how to effectively use Digitonin in experiments and optimizing experimental conditions, researchers can harness the benefits of this natural saponin for their research needs.
Digitonin (Reag. USP) is a natural saponin derived from the seeds of the plant Digitalis purpurea. It is widely used in biochemistry and cell biology research as a nonionic detergent that selectively permeabilizes the plasma membrane of cells. Digitonin forms complexes with cholesterol in the cell membrane, allowing for the release of cytoplasmic components while preserving the integrity of intracellular organelles.
What are the benefits of using Digitonin (Reag. USP) in research?
Digitonin (Reag. USP) offers several advantages in research applications. It is a gentle and effective detergent that can be used for the solubilization and isolation of membrane proteins without denaturing them. Digitonin also has the ability to selectively permeabilize the plasma membrane, making it a useful tool for studying the interactions between proteins within cells. Additionally, Digitonin is compatible with a wide range of biochemical assays, making it a versatile reagent for various research applications.
How is Digitonin (Reag. USP) typically used in experiments?
Digitonin (Reag. USP) is commonly used in experiments to permeabilize cells for the study of intracellular proteins and processes. Researchers typically prepare a digitonin solution at a specific concentration and treat cells with the detergent for a defined period of time. Digitonin selectively permeabilizes the plasma membrane, allowing researchers to investigate the localization, interaction, and function of intracellular proteins. Following treatment with digitonin, cells can be further analyzed using techniques such as immunofluorescence, western blotting, or enzyme activity assays.
Are there any specific considerations when using Digitonin (Reag. USP) in experiments?
When using Digitonin (Reag. USP) in experiments, researchers should consider several factors to ensure optimal results. It is important to determine the appropriate concentration of digitonin for permeabilizing cells based on the specific experimental requirements. Additionally, researchers should optimize the treatment time and conditions to minimize nonspecific effects on cellular components. It is also recommended to validate the integrity of intracellular organelles following digitonin treatment to ensure the preservation of cellular structures. Overall, careful experimental design and optimization are essential for successful applications of Digitonin in research.
In conclusion, Digitonin (Reag. USP) is a valuable reagent for studying membrane proteins and intracellular processes in research. Its gentle and selective permeabilization properties make it a versatile tool for a wide range of applications in biochemistry and cell biology. By understanding how to effectively use Digitonin in experiments and optimizing experimental conditions, researchers can harness the benefits of this natural saponin for their research needs.
