n-Dodecyl-ß-D-Maltoside BioChemica

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Name n-Dodecyl-ß-D-Maltoside BioChemica
CAS Number 69227-93-6
Molecular Formula C24H46O11
Molecular Weight 510.62 g/mol
Appearance Clear to slightly hazy, colorless to light yellow liquid
Solubility Soluble in water, ethanol, and chloroform
Storage Store at room temperature
Purity ≥ 99%
Usage Non-ionic detergent for membrane protein solubilization
Synonyms Dodecylmaltoside, Lauryl maltoside
Melting Point 48-51°C
Boiling Point 760 mmHg at 399.1°C
FAQ
What is n-Dodecyl-ß-D-Maltoside and how is it commonly used in biochemical research?

n-Dodecyl-ß-D-Maltoside, also known as DM, is a non-ionic detergent commonly used in biochemical research to solubilize and stabilize membrane proteins. This detergent is particularly useful for the extraction and purification of integral membrane proteins, including G-protein coupled receptors, ion channels, and transporters.

How does n-Dodecyl-ß-D-Maltoside aid in the solubilization of membrane proteins?

n-Dodecyl-ß-D-Maltoside has a hydrophilic head group composed of two maltose sugar units, making it water-soluble. The hydrophobic tail consisting of a dodecyl chain allows the detergent to associate with the hydrophobic regions of membrane proteins, disrupting the lipid bilayer and solubilizing the proteins in solution. This property makes n-Dodecyl-ß-D-Maltoside an effective detergent for isolating and studying membrane proteins.

What are the advantages of using n-Dodecyl-ß-D-Maltoside over other detergents for membrane protein research?

n-Dodecyl-ß-D-Maltoside offers several advantages over other detergents commonly used for membrane protein research. Firstly, it has a relatively mild detergent strength, allowing for the extraction of membrane proteins without denaturation or aggregation. Additionally, DM is known for its ability to maintain the structural integrity and native conformation of membrane proteins, making it suitable for downstream applications such as crystallization or functional studies. Furthermore, n-Dodecyl-ß-D-Maltoside is compatible with a wide range of buffers and conditions, offering flexibility in experimental design and optimization.

Are there any limitations or considerations when using n-Dodecyl-ß-D-Maltoside in biochemical research?

While n-Dodecyl-ß-D-Maltoside is a versatile detergent for membrane protein research, there are some limitations to consider when using this reagent. One potential drawback is the relatively high critical micelle concentration (CMC) of DM, which may require higher concentrations to effectively solubilize certain membrane proteins. It is also important to note that some membrane proteins may be sensitive to the presence of detergents, impacting their stability or activity. Researchers should carefully evaluate the compatibility of n-Dodecyl-ß-D-Maltoside with their specific protein of interest and optimize conditions accordingly.

How can researchers best incorporate n-Dodecyl-ß-D-Maltoside into their experimental workflow for membrane protein research?

To incorporate n-Dodecyl-ß-D-Maltoside into their experimental workflow for membrane protein research, researchers should first determine the optimal concentration of DM required for solubilization and stabilization of their target protein. This can be achieved through titration experiments to assess the detergent's effects on protein stability and activity. Additionally, researchers should consider the compatibility of DM with other reagents or techniques being used in their experiments, such as chromatography or biophysical assays. By carefully optimizing the use of n-Dodecyl-ß-D-Maltoside in their experimental workflow, researchers can maximize the yield and quality of membrane proteins for their biochemical studies.
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