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| Product Name | DNase I |
| Enzyme Type | Endonuclease |
| Source | Microbial origin |
| Molecular Weight | 32 kDa |
| Optimal Temperature | 37°C |
| Optimal Ph | pH 7.5 |
| Specificity | Cleaves double-stranded DNA at sites with accessible phosphate groups |
| Applications | DNA footprinting, RNA purification, removal of DNA from RNA samples |
| Storage Conditions | Store at -20°C |
| Unit Definition | One unit will produce an A260 of 1.0 in 30 minutes at 37°C using highly polymerized DNA as a substrate |
| Shipping Conditions | Shipped on dry ice |
FAQ
What is DNase I?
DNase I is an enzyme that breaks down DNA by hydrolyzing phosphodiester linkages, resulting in the degradation of DNA molecules into smaller fragments. This enzyme plays a crucial role in various molecular biology applications, particularly in the field of nucleic acid research.
How is DNase I used in research?
DNase I is commonly used in research applications to remove contaminating genomic DNA from RNA samples, thus ensuring accurate results in gene expression studies. It is also utilized in chromatin immunoprecipitation (ChIP) assays to digest chromosomal DNA and enrich specific DNA-protein interactions. Additionally, DNase I is employed in DNA footprinting experiments to study protein-DNA interactions at single-nucleotide resolution.
What are the benefits of using DNase I?
One of the main advantages of using DNase I is its specificity for DNA, which allows for precise degradation of DNA molecules without affecting RNA or proteins. This enzyme is also highly effective in removing DNA contamination from RNA samples, thereby improving the reliability of downstream applications such as RT-PCR and gene expression analysis. Furthermore, DNase I is widely compatible with a variety of experimental protocols and can be easily inactivated by heat or chelating agents, making it a versatile tool for researchers.
How should DNase I be stored and handled?
DNase I should be stored at -20°C to -80°C in a constant temperature freezer to maintain its stability and enzymatic activity. It is essential to avoid repeated freeze-thaw cycles, as this can lead to enzyme inactivation. When working with DNase I, it is recommended to prepare aliquots to minimize the risk of contamination and degradation. Additionally, good laboratory practices, such as wearing gloves and using sterile equipment, should be followed to prevent contamination of the enzyme and ensure reliable results in experiments.
What are the key considerations when choosing a DNase I product?
When selecting a DNase I product for research purposes, several factors should be taken into account, including the enzyme's activity level, purity, and compatibility with specific protocols. It is essential to choose a DNase I preparation with high specific activity to ensure efficient degradation of DNA molecules. The purity of the enzyme is also crucial, as contaminants can interfere with experimental results. Moreover, researchers should verify that the chosen DNase I product is suitable for their specific applications, such as RNA purification or chromatin digestion, to achieve optimal outcomes in their experiments.
DNase I is an enzyme that breaks down DNA by hydrolyzing phosphodiester linkages, resulting in the degradation of DNA molecules into smaller fragments. This enzyme plays a crucial role in various molecular biology applications, particularly in the field of nucleic acid research.
How is DNase I used in research?
DNase I is commonly used in research applications to remove contaminating genomic DNA from RNA samples, thus ensuring accurate results in gene expression studies. It is also utilized in chromatin immunoprecipitation (ChIP) assays to digest chromosomal DNA and enrich specific DNA-protein interactions. Additionally, DNase I is employed in DNA footprinting experiments to study protein-DNA interactions at single-nucleotide resolution.
What are the benefits of using DNase I?
One of the main advantages of using DNase I is its specificity for DNA, which allows for precise degradation of DNA molecules without affecting RNA or proteins. This enzyme is also highly effective in removing DNA contamination from RNA samples, thereby improving the reliability of downstream applications such as RT-PCR and gene expression analysis. Furthermore, DNase I is widely compatible with a variety of experimental protocols and can be easily inactivated by heat or chelating agents, making it a versatile tool for researchers.
How should DNase I be stored and handled?
DNase I should be stored at -20°C to -80°C in a constant temperature freezer to maintain its stability and enzymatic activity. It is essential to avoid repeated freeze-thaw cycles, as this can lead to enzyme inactivation. When working with DNase I, it is recommended to prepare aliquots to minimize the risk of contamination and degradation. Additionally, good laboratory practices, such as wearing gloves and using sterile equipment, should be followed to prevent contamination of the enzyme and ensure reliable results in experiments.
What are the key considerations when choosing a DNase I product?
When selecting a DNase I product for research purposes, several factors should be taken into account, including the enzyme's activity level, purity, and compatibility with specific protocols. It is essential to choose a DNase I preparation with high specific activity to ensure efficient degradation of DNA molecules. The purity of the enzyme is also crucial, as contaminants can interfere with experimental results. Moreover, researchers should verify that the chosen DNase I product is suitable for their specific applications, such as RNA purification or chromatin digestion, to achieve optimal outcomes in their experiments.
