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| Name | Lysozyme |
| Product Type | Enzyme |
| Source | Egg white |
| Purity | ≥ 95% |
| Molecular Weight | 14.3 kDa |
| Optimum Ph | 6.2 - 6.5 |
| Specific Activity | ≥ 30,000 units/mg |
| Storage Temperature | -20°C |
| Applications | Cell lysis, bacterial clearance, protein extraction |
| Form | Powder |
| Solubility | Soluble in water |
| Recommended Buffer | Tris-HCl, pH 7.0 |
| Specificity | Cleaves β-1,4 glycosidic bonds between N-acetyl muramic acid and N-acetylglucosamine residues in peptidoglycan |
| Unit Definition | One unit will cause a decrease in absorbance of 0.001 per minute at pH 6.24 at 25°C with Micrococcus lysodeikticus as substrate |
| Shipping Condition | Room temperature |
| Shelf Life | 2 years |
FAQ
What is lysozyme and how is it used in molecular biology?
Lysozyme is an enzyme that plays a crucial role in protecting organisms from harmful bacteria by breaking down their cell walls. In molecular biology, lysozyme is commonly used in various applications such as DNA extraction, protein purification, and bacterial transformation.
How does lysozyme aid in DNA extraction?
Lysozyme is used in DNA extraction procedures to break down bacterial cell walls, releasing the DNA for further analysis. By digesting the cell wall, lysozyme facilitates the extraction of high-quality DNA from bacterial cells.
What is the role of lysozyme in protein purification?
In protein purification, lysozyme is used to disrupt bacterial cell walls, releasing intracellular proteins for purification. By breaking down the cell wall, lysozyme enables researchers to isolate and purify target proteins from bacterial sources.
How does lysozyme facilitate bacterial transformation?
In bacterial transformation experiments, lysozyme is used to weaken the cell walls of bacteria, making them more permeable to foreign DNA. This allows researchers to introduce exogenous DNA into bacterial cells, facilitating genetic modifications and recombinant DNA technology.
What are the advantages of using lysozyme in molecular biology applications?
Lysozyme offers several advantages in molecular biology, including its ability to efficiently break down bacterial cell walls, its compatibility with a wide range of experimental protocols, and its cost-effective nature. Additionally, lysozyme is stable and easy to work with, making it a popular choice for researchers in the field.
Lysozyme is an enzyme that plays a crucial role in protecting organisms from harmful bacteria by breaking down their cell walls. In molecular biology, lysozyme is commonly used in various applications such as DNA extraction, protein purification, and bacterial transformation.
How does lysozyme aid in DNA extraction?
Lysozyme is used in DNA extraction procedures to break down bacterial cell walls, releasing the DNA for further analysis. By digesting the cell wall, lysozyme facilitates the extraction of high-quality DNA from bacterial cells.
What is the role of lysozyme in protein purification?
In protein purification, lysozyme is used to disrupt bacterial cell walls, releasing intracellular proteins for purification. By breaking down the cell wall, lysozyme enables researchers to isolate and purify target proteins from bacterial sources.
How does lysozyme facilitate bacterial transformation?
In bacterial transformation experiments, lysozyme is used to weaken the cell walls of bacteria, making them more permeable to foreign DNA. This allows researchers to introduce exogenous DNA into bacterial cells, facilitating genetic modifications and recombinant DNA technology.
What are the advantages of using lysozyme in molecular biology applications?
Lysozyme offers several advantages in molecular biology, including its ability to efficiently break down bacterial cell walls, its compatibility with a wide range of experimental protocols, and its cost-effective nature. Additionally, lysozyme is stable and easy to work with, making it a popular choice for researchers in the field.
