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| Name | Proteinase K - Solution |
| Brand | Thermo Fisher Scientific |
| Product Type | Enzyme solution |
| Formulation | Liquid |
| Concentration | 20 mg/mL |
| Storage Conditions | Store at -20°C |
| Applications | Protein digestion, DNA and RNA isolation |
| Enzyme Source | Genetically modified Tritirachium album |
| Purity | ≥95% |
| Unit Definition | One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per min |
| Molecular Weight | 29.2 kDa |
| Activity | ≥30 U/mg |
FAQ
What is Proteinase K?
Proteinase K is a highly active serine protease that is widely used in molecular biology applications. It cleaves proteins at the carboxylic acid side of hydrophobic, aromatic, or S- amino acids. Proteinase K is stable over a wide range of temperatures and pH levels, making it ideal for use in various experimental conditions.
What are the benefits of using Proteinase K Solution?
Proteinase K Solution offers a convenient and ready-to-use format for researchers. It eliminates the need for additional preparation steps and ensures consistent results. The solution is free of contaminants that may interfere with downstream applications, providing reliable and reproducible performance.
How is Proteinase K Solution commonly used in research?
Proteinase K Solution is commonly used for DNA and RNA purification, protein analysis, and molecular cloning. It is often employed to degrade proteins in samples, allowing for the isolation of nucleic acids or the study of protein-protein interactions. Proteinase K Solution can also be used to remove enzymes or antibodies from nucleic acid hybridization membranes.
What are the storage recommendations for Proteinase K Solution?
Proteinase K Solution should be stored at -20°C to -80°C upon receipt. Once thawed, it can be stored at 4°C for short-term use. Avoid repeated freeze-thaw cycles to maintain the stability and activity of the enzyme. It is recommended to aliquot the solution into smaller volumes to prevent contamination and ensure long-term storage.
What precautions should be taken when working with Proteinase K Solution?
When working with Proteinase K Solution, it is important to wear appropriate personal protective equipment, such as gloves and lab coats. Avoid contact with skin, eyes, and mucous membranes, as the enzyme may cause irritation. Follow proper guidelines for handling and disposal of chemical reagents to ensure safety in the laboratory.
Proteinase K is a highly active serine protease that is widely used in molecular biology applications. It cleaves proteins at the carboxylic acid side of hydrophobic, aromatic, or S- amino acids. Proteinase K is stable over a wide range of temperatures and pH levels, making it ideal for use in various experimental conditions.
What are the benefits of using Proteinase K Solution?
Proteinase K Solution offers a convenient and ready-to-use format for researchers. It eliminates the need for additional preparation steps and ensures consistent results. The solution is free of contaminants that may interfere with downstream applications, providing reliable and reproducible performance.
How is Proteinase K Solution commonly used in research?
Proteinase K Solution is commonly used for DNA and RNA purification, protein analysis, and molecular cloning. It is often employed to degrade proteins in samples, allowing for the isolation of nucleic acids or the study of protein-protein interactions. Proteinase K Solution can also be used to remove enzymes or antibodies from nucleic acid hybridization membranes.
What are the storage recommendations for Proteinase K Solution?
Proteinase K Solution should be stored at -20°C to -80°C upon receipt. Once thawed, it can be stored at 4°C for short-term use. Avoid repeated freeze-thaw cycles to maintain the stability and activity of the enzyme. It is recommended to aliquot the solution into smaller volumes to prevent contamination and ensure long-term storage.
What precautions should be taken when working with Proteinase K Solution?
When working with Proteinase K Solution, it is important to wear appropriate personal protective equipment, such as gloves and lab coats. Avoid contact with skin, eyes, and mucous membranes, as the enzyme may cause irritation. Follow proper guidelines for handling and disposal of chemical reagents to ensure safety in the laboratory.
