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| Product Name | RNase A (DNase-free) |
| Product Type | Enzyme |
| Source | Recombinant |
| Purity | ≥99% |
| Activity | DNase-free |
| Form | Lyophilized powder |
| Storage Condition | Store at -20°C |
| Applications | RNA purification, in vitro transcription, molecular biology research |
| Molecular Weight | 13.7 kDa |
| Unit Definition | One unit will hydrolyze 1nmol of RNA at pH 5.0 at 37°C in 1 hour |
FAQ
What is RNase A (DNase-free) and how is it used in research?
RNase A is an enzyme that specifically cleaves RNA, making it a valuable tool for researchers working with RNA samples. DNase-free RNase A is free of any contaminating DNase, ensuring the purity of RNA samples during experiments.
How does RNase A (DNase-free) differ from regular RNase A?
Regular RNase A can potentially degrade DNA in addition to RNA, which can be problematic for researchers working with both nucleic acids. DNase-free RNase A is specifically purified to remove any DNase activity, making it suitable for experiments where DNA integrity is crucial.
What are the applications of using DNase-free RNase A in research?
DNase-free RNase A is commonly used in RNA extraction protocols to remove contaminating RNA, ensuring the purity of RNA samples for downstream applications such as RT-PCR, RNA sequencing, and gene expression analysis. It can also be used to degrade unwanted RNA in hybridization experiments or to control RNA integrity in various molecular biology techniques.
How is DNase-free RNase A typically added to RNA samples?
DNase-free RNase A is usually added to RNA samples at a specific concentration and incubated for a defined period of time to ensure complete RNA degradation. The enzyme can be inactivated by heat or specific inhibitors after the desired RNA cleavage has been achieved, preventing further degradation of the RNA sample.
Are there any precautions researchers should take when working with DNase-free RNase A?
Researchers should handle DNase-free RNase A with care to avoid contamination of RNA samples with RNases from skin or other sources. It is important to work in a designated RNA-free area and use RNase-free reagents and equipment to prevent degradation of RNA samples during experiments. Additionally, researchers should follow manufacturer guidelines for proper storage and handling of DNase-free RNase A to maintain its activity and effectiveness in RNA cleavage.
RNase A is an enzyme that specifically cleaves RNA, making it a valuable tool for researchers working with RNA samples. DNase-free RNase A is free of any contaminating DNase, ensuring the purity of RNA samples during experiments.
How does RNase A (DNase-free) differ from regular RNase A?
Regular RNase A can potentially degrade DNA in addition to RNA, which can be problematic for researchers working with both nucleic acids. DNase-free RNase A is specifically purified to remove any DNase activity, making it suitable for experiments where DNA integrity is crucial.
What are the applications of using DNase-free RNase A in research?
DNase-free RNase A is commonly used in RNA extraction protocols to remove contaminating RNA, ensuring the purity of RNA samples for downstream applications such as RT-PCR, RNA sequencing, and gene expression analysis. It can also be used to degrade unwanted RNA in hybridization experiments or to control RNA integrity in various molecular biology techniques.
How is DNase-free RNase A typically added to RNA samples?
DNase-free RNase A is usually added to RNA samples at a specific concentration and incubated for a defined period of time to ensure complete RNA degradation. The enzyme can be inactivated by heat or specific inhibitors after the desired RNA cleavage has been achieved, preventing further degradation of the RNA sample.
Are there any precautions researchers should take when working with DNase-free RNase A?
Researchers should handle DNase-free RNase A with care to avoid contamination of RNA samples with RNases from skin or other sources. It is important to work in a designated RNA-free area and use RNase-free reagents and equipment to prevent degradation of RNA samples during experiments. Additionally, researchers should follow manufacturer guidelines for proper storage and handling of DNase-free RNase A to maintain its activity and effectiveness in RNA cleavage.
