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| Name | Trypsin 1 : 250 from porcine pancreas |
| Type | Enzyme |
| Source | Porcine pancreas |
| Activity | 1: 250 |
| Purity | ≥ 250 BAEE units/mg protein |
| Form | Lyophilized powder |
| Storage | Store at -20°C |
| Solubility | Soluble in water |
| Specificity | Cleaves peptide bonds at the carboxyl side of lysine and arginine residues |
| Applications | Protein digestion, peptide mapping, protein sequencing |
| Molecular Weight | Approximately 23 kDa |
| PH Optimum | pH 7.5-9.0 |
FAQ
What is Trypsin 1:250 from porcine pancreas?
Trypsin 1:250 from porcine pancreas is a high-quality enzyme product derived from the pancreas of pigs. It contains trypsin, a protease enzyme that plays a crucial role in the breakdown of proteins during digestion.
How is Trypsin 1:250 used in laboratory applications?
Trypsin 1:250 is commonly used in cell culture applications to detach and dissociate adherent cells from the culture vessel. It can also be used for the isolation of primary cells, subculturing, and other cell biology experiments.
What are the advantages of using Trypsin 1:250 from porcine pancreas?
One of the main advantages of using Trypsin 1:250 is its high specific activity, which ensures efficient cell detachment and minimal cell damage. Additionally, it is a cost-effective option for researchers conducting cell culture experiments.
Are there any precautions that should be taken when using Trypsin 1:250?
It is important to handle Trypsin 1:250 with care and follow proper safety protocols, as it is an enzyme that can be harmful if ingested or inhaled. Users should wear appropriate personal protective equipment and work in a well-ventilated area when handling the product.
How should Trypsin 1:250 be stored?
Trypsin 1:250 should be stored in a tightly sealed container at -20°C to maintain its stability and enzymatic activity. It is important to avoid repeated freeze-thaw cycles, as this can degrade the enzyme and reduce its effectiveness.
Trypsin 1:250 from porcine pancreas is a high-quality enzyme product derived from the pancreas of pigs. It contains trypsin, a protease enzyme that plays a crucial role in the breakdown of proteins during digestion.
How is Trypsin 1:250 used in laboratory applications?
Trypsin 1:250 is commonly used in cell culture applications to detach and dissociate adherent cells from the culture vessel. It can also be used for the isolation of primary cells, subculturing, and other cell biology experiments.
What are the advantages of using Trypsin 1:250 from porcine pancreas?
One of the main advantages of using Trypsin 1:250 is its high specific activity, which ensures efficient cell detachment and minimal cell damage. Additionally, it is a cost-effective option for researchers conducting cell culture experiments.
Are there any precautions that should be taken when using Trypsin 1:250?
It is important to handle Trypsin 1:250 with care and follow proper safety protocols, as it is an enzyme that can be harmful if ingested or inhaled. Users should wear appropriate personal protective equipment and work in a well-ventilated area when handling the product.
How should Trypsin 1:250 be stored?
Trypsin 1:250 should be stored in a tightly sealed container at -20°C to maintain its stability and enzymatic activity. It is important to avoid repeated freeze-thaw cycles, as this can degrade the enzyme and reduce its effectiveness.
