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| Product Name | Trypsin from bovine pancreas |
| Source | Bovine pancreas |
| Enzyme Type | Protease |
| Activity | ≥2500 USP units/mg solid |
| Appearance | White to off-white powder |
| Solubility | Soluble in water |
| Stability | Stable at room temperature for short term storage |
| Storage | Store at -20°C |
| Purity | ≥95% |
| Usage | Research, biotechnology applications |
| Molecular Weight | Approximately 23 kDa |
FAQ
What is Trypsin from bovine pancreas and how is it used?
Trypsin from bovine pancreas is a digestive enzyme that breaks down proteins into smaller peptides. It is commonly used in cell culture applications to dissociate adherent cells and tissues into a single-cell suspension for further analysis or experimentation.
How does Trypsin work in dissociating cells and tissues?
Trypsin works by cleaving peptide bonds in proteins, specifically targeting the amino acids lysine and arginine. This cleavage disrupts the interactions between cells and the extracellular matrix, allowing them to detach from the surface and form a single-cell suspension for further processing.
What are the advantages of using Trypsin for cell dissociation compared to other methods?
Trypsin is a highly effective enzyme that works quickly to dissociate cells and tissues without damaging cell membranes or causing excessive cell death. It is a gentle and efficient method that is widely used in cell culture laboratories for routine cell passaging and experiments.
Are there any precautions or considerations to keep in mind when using Trypsin for cell dissociation?
It is important to carefully optimize the concentration and incubation time of Trypsin to avoid over-digestion of cells, which can lead to decreased cell viability and functionality. Additionally, it is crucial to use Trypsin-free media or inhibitors to neutralize the enzyme activity once cell detachment is achieved.
How should Trypsin from bovine pancreas be stored and handled to maintain its efficacy?
Trypsin should be stored at -20°C in aliquots to prevent repeated freeze-thaw cycles that can degrade enzyme activity. It is important to follow manufacturer recommendations for reconstitution and storage to ensure optimal performance. Additionally, proper handling techniques, such as using sterile techniques and working in a laminar flow hood, should be employed to prevent contamination and maintain cell culture sterility.
Trypsin from bovine pancreas is a digestive enzyme that breaks down proteins into smaller peptides. It is commonly used in cell culture applications to dissociate adherent cells and tissues into a single-cell suspension for further analysis or experimentation.
How does Trypsin work in dissociating cells and tissues?
Trypsin works by cleaving peptide bonds in proteins, specifically targeting the amino acids lysine and arginine. This cleavage disrupts the interactions between cells and the extracellular matrix, allowing them to detach from the surface and form a single-cell suspension for further processing.
What are the advantages of using Trypsin for cell dissociation compared to other methods?
Trypsin is a highly effective enzyme that works quickly to dissociate cells and tissues without damaging cell membranes or causing excessive cell death. It is a gentle and efficient method that is widely used in cell culture laboratories for routine cell passaging and experiments.
Are there any precautions or considerations to keep in mind when using Trypsin for cell dissociation?
It is important to carefully optimize the concentration and incubation time of Trypsin to avoid over-digestion of cells, which can lead to decreased cell viability and functionality. Additionally, it is crucial to use Trypsin-free media or inhibitors to neutralize the enzyme activity once cell detachment is achieved.
How should Trypsin from bovine pancreas be stored and handled to maintain its efficacy?
Trypsin should be stored at -20°C in aliquots to prevent repeated freeze-thaw cycles that can degrade enzyme activity. It is important to follow manufacturer recommendations for reconstitution and storage to ensure optimal performance. Additionally, proper handling techniques, such as using sterile techniques and working in a laminar flow hood, should be employed to prevent contamination and maintain cell culture sterility.
