Eosin Yellowish (C.I. 45380) for clinical diagnosis

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Name Eosin Yellowish
IUPAC Name 2',4',5'-trichloro-4-biphenylylazo-4'-hydroxybenzenesulfonic acid
CAS Number 17372-87-1
Molecular Formula C20H8Cl3NO5S
Molecular Weight 494.71 g/mol
Appearance Yellow powder
Solubility Slightly soluble in water
Usage Commonly used as a histological stain in clinical diagnosis
Stability Stable under normal conditions
Storage Store in a cool, dry place
Handling Avoid inhalation and skin contact
Toxicity Low toxicity if ingested, may cause skin irritation
FAQ
What is Eosin Yellowish (C.I. 45380) and how is it used for clinical diagnosis?
Eosin Yellowish (C.I. 45380) is a synthetic dye that is commonly used in histology and pathology for staining biological samples. It is particularly useful for highlighting structures such as collagen fibers, erythrocytes, and muscle striations in tissue sections. This dye can help pathologists and researchers visualize cellular structures and identify abnormalities in tissues, making it a valuable tool for clinical diagnosis.

How does Eosin Yellowish (C.I. 45380) help in the differentiation of cells?
Eosin Yellowish (C.I. 45380) is an acidic dye that binds to basic structures in cells, such as proteins and amino acids. This staining process allows for the differentiation of cells and tissues based on their color and texture, making it easier to identify specific cell types and structures. By staining with Eosin Yellowish, researchers can distinguish between different cell populations and assess the presence of abnormalities or pathological changes.

What are the advantages of using Eosin Yellowish (C.I. 45380) for clinical diagnosis?
One of the main advantages of using Eosin Yellowish (C.I. 45380) for clinical diagnosis is its ability to provide high contrast staining of cellular structures. This dye allows for clear visualization of tissues under a microscope, enabling pathologists to make accurate assessments of cellular morphology and organization. Additionally, Eosin Yellowish is easy to use and compatible with various staining protocols, making it a versatile tool for different diagnostic applications.

How can Eosin Yellowish (C.I. 45380) be incorporated into routine laboratory procedures?
Eosin Yellowish (C.I. 45380) can be easily incorporated into routine laboratory procedures by following standard staining protocols for histological and cytological specimens. Typically, Eosin Yellowish is applied after hematoxylin staining to provide contrast and highlight specific cellular structures. It is important to follow recommended guidelines for staining times and concentrations to achieve optimal results. By integrating Eosin Yellowish into routine laboratory procedures, researchers and pathologists can improve the quality and accuracy of their diagnostic assessments.

What are some tips for using Eosin Yellowish (C.I. 45380) effectively in clinical diagnosis?
When using Eosin Yellowish (C.I. 45380) for clinical diagnosis, it is important to ensure proper handling and storage of the dye to maintain its stability and efficacy. Additionally, it is recommended to perform regular quality control checks to monitor staining consistency and reproducibility. It is also essential to follow standardized staining protocols and adjust staining times and concentrations as needed based on the specific requirements of the samples being analyzed. By following these tips, researchers and pathologists can maximize the utility of Eosin Yellowish for accurate and reliable clinical diagnosis.
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