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| Name | Fehling's B Reagent |
| Use | clinical diagnosis |
| Type | chemical reagent |
| Color | blue |
| Composition | copper sulfate, potassium sodium tartrate, sodium hydroxide |
| Application | used to test for the presence of reducing sugars in urine or blood samples |
| Reaction | forms a brick red precipitate when heated with reducing sugars |
| Preparation | two solutions (A and B) mixed in equal volumes before use |
| Storage | store in a cool, dry place away from direct sunlight |
| Shelf Life | 6 months |
| Sensitivity | detects reducing sugars at concentrations as low as 0.1% |
| Specificity | reacts with reducing sugars but not with non-reducing sugars |
FAQ
What is Fehling's B Reagent used for in clinical diagnosis?
Fehling's B Reagent is commonly used in laboratory settings for the detection of reducing sugars, most notably in the testing for glucose in urine samples. This reagent helps in the identification of various health conditions such as diabetes, as the presence of glucose in urine can indicate high blood sugar levels.
How does Fehling's B Reagent work?
Fehling's B Reagent works based on the principle of reducing sugars. When a reducing sugar such as glucose is present in a sample, it reacts with the copper ions in the reagent. This reaction results in the reduction of copper (II) ions to copper (I) ions, forming a red precipitate of cuprous oxide. The intensity of the red color formation is then used to determine the concentration of reducing sugars in the sample.
What are the advantages of using Fehling's B Reagent for clinical diagnosis?
One of the key advantages of using Fehling's B Reagent is its specificity for reducing sugars, making it a reliable method for identifying glucose in urine samples. Additionally, this reagent is easy to use and provides rapid results, making it a valuable tool for healthcare professionals in diagnosing conditions such as diabetes.
Are there any limitations or considerations when using Fehling's B Reagent?
While Fehling's B Reagent is effective for detecting reducing sugars, it is important to note that it may not be specific for all types of sugars. Some non-reducing sugars may not react with this reagent, leading to potential false-negative results. It is also essential to follow proper procedure and ensure accurate measurements to obtain reliable results with this reagent.
How is Fehling's B Reagent prepared and stored?
Fehling's B Reagent is typically prepared by combining equal volumes of Fehling's A and Fehling's B solutions. The reagent should be stored in a cool, dark place away from direct light and extreme temperatures to maintain its stability. It is important to check the expiry date and discard any expired or deteriorated reagent to ensure accurate results in clinical diagnosis.
Fehling's B Reagent is commonly used in laboratory settings for the detection of reducing sugars, most notably in the testing for glucose in urine samples. This reagent helps in the identification of various health conditions such as diabetes, as the presence of glucose in urine can indicate high blood sugar levels.
How does Fehling's B Reagent work?
Fehling's B Reagent works based on the principle of reducing sugars. When a reducing sugar such as glucose is present in a sample, it reacts with the copper ions in the reagent. This reaction results in the reduction of copper (II) ions to copper (I) ions, forming a red precipitate of cuprous oxide. The intensity of the red color formation is then used to determine the concentration of reducing sugars in the sample.
What are the advantages of using Fehling's B Reagent for clinical diagnosis?
One of the key advantages of using Fehling's B Reagent is its specificity for reducing sugars, making it a reliable method for identifying glucose in urine samples. Additionally, this reagent is easy to use and provides rapid results, making it a valuable tool for healthcare professionals in diagnosing conditions such as diabetes.
Are there any limitations or considerations when using Fehling's B Reagent?
While Fehling's B Reagent is effective for detecting reducing sugars, it is important to note that it may not be specific for all types of sugars. Some non-reducing sugars may not react with this reagent, leading to potential false-negative results. It is also essential to follow proper procedure and ensure accurate measurements to obtain reliable results with this reagent.
How is Fehling's B Reagent prepared and stored?
Fehling's B Reagent is typically prepared by combining equal volumes of Fehling's A and Fehling's B solutions. The reagent should be stored in a cool, dark place away from direct light and extreme temperatures to maintain its stability. It is important to check the expiry date and discard any expired or deteriorated reagent to ensure accurate results in clinical diagnosis.
