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| Name | Hematoxylin 1-hydrate (C.I. 75290) |
| Synonyms | Natural Black 1, Hematein |
| CAS Number | 517-28-2 |
| Formula | C16H14O6 |
| Molar Mass | 302.28 g/mol |
| Appearance | Purple crystalline powder |
| Solubility | Soluble in alcohol, alkaline solutions |
| Storage | Store in a cool, dry place away from light |
| Usage | Used as a nuclear and cytoplasmic stain in histology |
| Stability | Stable under recommended storage conditions |
| Purity | ≥ 88% |
| Handling | Wear appropriate protective equipment when handling |
FAQ
1. What is Hematoxylin 1-hydrate (C.I. 75290) and how is it used in clinical
diagnosis?
Hematoxylin 1-hydrate is a common histological stain used in clinical diagnosis to visualize cellular structures, particularly nuclei, in tissue samples. It is commonly employed in conjunction with eosin to create the widely-used H&E (Hematoxylin and Eosin) stain, which provides differential staining of cell nuclei and cytoplasm, allowing for the visualization of various cellular components and structures. Hematoxylin stains nuclei blue-purple, highlighting the cellular architecture and aiding in the identification of abnormalities or specific cell types under a microscope.
2. How does Hematoxylin 1-hydrate enhance the accuracy of clinical diagnoses?
By selectively staining cell nuclei, Hematoxylin 1-hydrate provides contrast and clarity to tissue samples, making it easier for pathologists and clinicians to identify specific cellular structures, abnormalities, and pathological changes. This enhanced visibility allows for more accurate and precise diagnoses of various diseases, conditions, and disorders based on the morphological characteristics of the cells present in the tissue sample. Hematoxylin staining is an essential tool in histopathology and plays a crucial role in the accurate interpretation of tissue specimens.
3. What are the key benefits of using Hematoxylin 1-hydrate for clinical diagnosis?
One of the primary benefits of using Hematoxylin 1-hydrate for clinical diagnosis is its ability to selectively highlight cell nuclei, aiding in the identification of specific cell types, morphological features, and pathological changes in tissue samples. This enhanced visibility facilitates accurate diagnoses and helps pathologists and clinicians make informed decisions regarding patient care. Additionally, Hematoxylin staining is a reliable and cost-effective method for visualizing cellular structures, making it an essential tool in diagnostic pathology.
4. How is Hematoxylin 1-hydrate prepared and applied in the laboratory for clinical diagnosis?
Hematoxylin 1-hydrate is typically prepared as a staining solution by dissolving the dye in an appropriate solvent, such as ethanol or water, to create a working solution with a specific concentration. The staining protocol may vary depending on the type of tissue sample and the desired results, but in general, the tissue sections are immersed in the Hematoxylin solution for a specific period, followed by counterstaining with eosin to visualize cytoplasmic structures. The stained slides are then examined under a microscope to evaluate the cellular morphology and make a diagnosis based on the staining patterns observed.
5. Can Hematoxylin 1-hydrate be used in conjunction with other stains for additional diagnostic information?
Yes, Hematoxylin 1-hydrate is often used in combination with other histological stains, such as eosin, to provide additional diagnostic information. Different stains can highlight various cellular components and structures, allowing pathologists to obtain a comprehensive view of the tissue sample and make a more accurate diagnosis. For example, the PAS (Periodic Acid-Schiff) stain can be used in conjunction with Hematoxylin to detect glycogen and mucin in tissue samples, providing valuable information for the diagnosis of certain diseases. By combining different staining techniques, pathologists can enhance the diagnostic capabilities of Hematoxylin and obtain a more detailed understanding of the tissue architecture and composition.
Hematoxylin 1-hydrate is a common histological stain used in clinical diagnosis to visualize cellular structures, particularly nuclei, in tissue samples. It is commonly employed in conjunction with eosin to create the widely-used H&E (Hematoxylin and Eosin) stain, which provides differential staining of cell nuclei and cytoplasm, allowing for the visualization of various cellular components and structures. Hematoxylin stains nuclei blue-purple, highlighting the cellular architecture and aiding in the identification of abnormalities or specific cell types under a microscope.
2. How does Hematoxylin 1-hydrate enhance the accuracy of clinical diagnoses?
By selectively staining cell nuclei, Hematoxylin 1-hydrate provides contrast and clarity to tissue samples, making it easier for pathologists and clinicians to identify specific cellular structures, abnormalities, and pathological changes. This enhanced visibility allows for more accurate and precise diagnoses of various diseases, conditions, and disorders based on the morphological characteristics of the cells present in the tissue sample. Hematoxylin staining is an essential tool in histopathology and plays a crucial role in the accurate interpretation of tissue specimens.
3. What are the key benefits of using Hematoxylin 1-hydrate for clinical diagnosis?
One of the primary benefits of using Hematoxylin 1-hydrate for clinical diagnosis is its ability to selectively highlight cell nuclei, aiding in the identification of specific cell types, morphological features, and pathological changes in tissue samples. This enhanced visibility facilitates accurate diagnoses and helps pathologists and clinicians make informed decisions regarding patient care. Additionally, Hematoxylin staining is a reliable and cost-effective method for visualizing cellular structures, making it an essential tool in diagnostic pathology.
4. How is Hematoxylin 1-hydrate prepared and applied in the laboratory for clinical diagnosis?
Hematoxylin 1-hydrate is typically prepared as a staining solution by dissolving the dye in an appropriate solvent, such as ethanol or water, to create a working solution with a specific concentration. The staining protocol may vary depending on the type of tissue sample and the desired results, but in general, the tissue sections are immersed in the Hematoxylin solution for a specific period, followed by counterstaining with eosin to visualize cytoplasmic structures. The stained slides are then examined under a microscope to evaluate the cellular morphology and make a diagnosis based on the staining patterns observed.
5. Can Hematoxylin 1-hydrate be used in conjunction with other stains for additional diagnostic information?
Yes, Hematoxylin 1-hydrate is often used in combination with other histological stains, such as eosin, to provide additional diagnostic information. Different stains can highlight various cellular components and structures, allowing pathologists to obtain a comprehensive view of the tissue sample and make a more accurate diagnosis. For example, the PAS (Periodic Acid-Schiff) stain can be used in conjunction with Hematoxylin to detect glycogen and mucin in tissue samples, providing valuable information for the diagnosis of certain diseases. By combining different staining techniques, pathologists can enhance the diagnostic capabilities of Hematoxylin and obtain a more detailed understanding of the tissue architecture and composition.
