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| Name | Bis-Tris for buffer solutions |
| Purity | high purity |
| Form | powder |
| Solubility | high solubility in water |
| PH Range | 5.8 - 7.3 |
| Molecular Formula | C8H19NO5S |
| Molecular Weight | 249.31 g/mol |
| Storage Conditions | store at room temperature |
| Applications | used for protein electrophoresis and protein crystallization |
| Shelf Life | stable for at least 2 years when stored properly |
| Appearance | white to off-white crystalline powder |
| Melting Point | 176 - 178°C |
| Boiling Point | decomposes |
| Density | 1.280 g/cm3 |
| CAS Number | 6976-37-0 |
| EC Number | 230-392-0 |
FAQ
What is Bis-Tris?
Bis-Tris is a buffering agent commonly used in biochemical research to maintain the pH of solutions within a specific range. It is particularly effective in the pH range of 5.8-7.8, making it ideal for many laboratory applications.
How does Bis-Tris work as a buffer?
Bis-Tris functions as a buffer by accepting or releasing hydrogen ions in response to changes in pH. This helps to stabilize the pH of a solution, making it resistant to large fluctuations in acidity or alkalinity.
What are the advantages of using Bis-Tris for buffer solutions?
One of the main advantages of using Bis-Tris is its ability to maintain a stable pH in a specific range, which is crucial for many biochemical experiments. It is also highly soluble in water and has low UV absorbance, making it suitable for use in spectrophotometric assays.
How do I prepare a buffer solution using Bis-Tris?
To prepare a buffer solution using Bis-Tris, simply dissolve the desired amount of Bis-Tris powder in water and adjust the pH as needed using a strong acid or base. It is important to carefully follow the instructions provided with the Bis-Tris product to ensure the proper formulation of the buffer solution.
Are there any precautions to consider when using Bis-Tris for buffer solutions?
While Bis-Tris is generally considered safe to use in laboratory settings, it is important to handle it with care and wear appropriate personal protective equipment. Avoid inhaling the powder or getting it in contact with your skin or eyes. Additionally, always store Bis-Tris in a dry and cool environment to prevent degradation.
Bis-Tris is a buffering agent commonly used in biochemical research to maintain the pH of solutions within a specific range. It is particularly effective in the pH range of 5.8-7.8, making it ideal for many laboratory applications.
How does Bis-Tris work as a buffer?
Bis-Tris functions as a buffer by accepting or releasing hydrogen ions in response to changes in pH. This helps to stabilize the pH of a solution, making it resistant to large fluctuations in acidity or alkalinity.
What are the advantages of using Bis-Tris for buffer solutions?
One of the main advantages of using Bis-Tris is its ability to maintain a stable pH in a specific range, which is crucial for many biochemical experiments. It is also highly soluble in water and has low UV absorbance, making it suitable for use in spectrophotometric assays.
How do I prepare a buffer solution using Bis-Tris?
To prepare a buffer solution using Bis-Tris, simply dissolve the desired amount of Bis-Tris powder in water and adjust the pH as needed using a strong acid or base. It is important to carefully follow the instructions provided with the Bis-Tris product to ensure the proper formulation of the buffer solution.
Are there any precautions to consider when using Bis-Tris for buffer solutions?
While Bis-Tris is generally considered safe to use in laboratory settings, it is important to handle it with care and wear appropriate personal protective equipment. Avoid inhaling the powder or getting it in contact with your skin or eyes. Additionally, always store Bis-Tris in a dry and cool environment to prevent degradation.
