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Product Name | CHES for Buffer Solutions |
Product Type | Chemical reagent |
Packaging Type | Bottle |
Quantity | 500 grams |
Purity | High purity |
Solubility | Soluble in water |
Storage Conditions | Store in a cool, dry place |
Shelf Life | 2 years |
Used For | Preparing buffer solutions for research and analytical applications |
PH Range | pH 8.6-10.0 |
Molecular Weight | 229.33 g/mol |
Melting Point | 295-299°C |
Boiling Point | N/A |
Appearance | White crystalline powder |
Density | 1.213 g/cm3 |
Specificity | Good buffering capacity in the specified pH range |
Applications | Biochemistry, molecular biology, and other scientific research |
Compatibility | Compatible with a wide range of buffer components |
Manufacturing Source | Synthetic production |
FAQ
What are CHES buffer solutions?
CHES buffer solutions are specially formulated solutions used in biochemistry and molecular biology research for maintaining a stable pH environment. CHES stands for N-cyclohexyl-2-aminoethanesulfonic acid, a zwitterionic buffer that is effective over a wide range of pH levels.
Why are CHES buffer solutions popular in research laboratories?
CHES buffer solutions are popular in research laboratories because of their ability to maintain a stable pH environment over a wide range of temperatures. They are also known for their high buffering capacity, making them ideal for experiments that require precise pH control.
How are CHES buffer solutions used in experiments?
CHES buffer solutions are commonly used in a variety of experiments, including protein purification, enzyme assays, and molecular biology techniques such as PCR and gel electrophoresis. They are used to maintain a specific pH environment that is conducive to the experiment being conducted.
What are the advantages of using CHES buffer solutions over other buffers?
CHES buffer solutions offer several advantages over other buffers, including a wide buffering range (pH 8.6-10.0), high solubility, and low UV absorbance. They are also less likely to interfere with sensitive assays, making them a popular choice for many researchers.
Are there any limitations to using CHES buffer solutions?
While CHES buffer solutions are highly versatile and effective in many experiments, they may not be suitable for all applications. Researchers should consider factors such as the desired pH range and compatibility with other reagents before choosing to use CHES buffers.
CHES buffer solutions are specially formulated solutions used in biochemistry and molecular biology research for maintaining a stable pH environment. CHES stands for N-cyclohexyl-2-aminoethanesulfonic acid, a zwitterionic buffer that is effective over a wide range of pH levels.
Why are CHES buffer solutions popular in research laboratories?
CHES buffer solutions are popular in research laboratories because of their ability to maintain a stable pH environment over a wide range of temperatures. They are also known for their high buffering capacity, making them ideal for experiments that require precise pH control.
How are CHES buffer solutions used in experiments?
CHES buffer solutions are commonly used in a variety of experiments, including protein purification, enzyme assays, and molecular biology techniques such as PCR and gel electrophoresis. They are used to maintain a specific pH environment that is conducive to the experiment being conducted.
What are the advantages of using CHES buffer solutions over other buffers?
CHES buffer solutions offer several advantages over other buffers, including a wide buffering range (pH 8.6-10.0), high solubility, and low UV absorbance. They are also less likely to interfere with sensitive assays, making them a popular choice for many researchers.
Are there any limitations to using CHES buffer solutions?
While CHES buffer solutions are highly versatile and effective in many experiments, they may not be suitable for all applications. Researchers should consider factors such as the desired pH range and compatibility with other reagents before choosing to use CHES buffers.