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| Name | Guanidine Hydrochloride |
| Category | Molecular Biology |
| Form | Powder |
| Purity | ≥98% |
| Solubility | Water |
| Storage | Store at room temperature |
| Molecular Weight | 95.53 g/mol |
| Melting Point | 182-185°C |
| Boiling Point | 118°C (0.8mmHg) |
| Appearance | White crystalline powder |
| Cas Number | 50-01-1 |
| MdL Number | MFCD00013017 |
| PubChem CID | 5740 |
FAQ
What is Guanidine Hydrochloride and what are its applications in molecular
biology?
Guanidine Hydrochloride is a chaotropic agent commonly used in molecular biology. It is utilized for the denaturation of proteins and nucleic acids, aiding in the disruption of hydrogen bonds and hydrophobic interactions. This reagent is essential for various applications such as protein purification, DNA and RNA isolation, and protein refolding.
How does Guanidine Hydrochloride help in protein purification?
Guanidine Hydrochloride plays a crucial role in protein purification by denaturing the proteins, disrupting their native conformation, and promoting solubilization. This chaotropic agent helps to unfold the proteins, exposing the hydrophobic regions, which facilitates the removal of contaminants and allows for the isolation of the target protein.
Can Guanidine Hydrochloride be used for DNA and RNA isolation?
Yes, Guanidine Hydrochloride is commonly used for DNA and RNA isolation due to its ability to denature macromolecules and inhibit nuclease activity. By disrupting the structure of nucleic acids, Guanidine Hydrochloride helps to release them from cellular components and maintain their stability during extraction procedures.
How can Guanidine Hydrochloride aid in protein refolding?
Protein refolding is a challenging process that requires denaturation and renaturation of the protein to achieve its native conformation. Guanidine Hydrochloride is often used in protein refolding protocols as a denaturant to unfold misfolded proteins and facilitate their correct refolding into the biologically active form.
What are some best practices for using Guanidine Hydrochloride in molecular biology experiments?
When working with Guanidine Hydrochloride, it is important to handle the reagent with caution as it can be irritating to the skin, eyes, and respiratory tract. Use appropriate personal protective equipment such as gloves and goggles. Additionally, always prepare solutions of Guanidine Hydrochloride in a well-ventilated area and avoid inhaling the vapors. Store the reagent at the recommended temperature and protect it from light to maintain its stability for optimal performance in molecular biology applications.
Guanidine Hydrochloride is a chaotropic agent commonly used in molecular biology. It is utilized for the denaturation of proteins and nucleic acids, aiding in the disruption of hydrogen bonds and hydrophobic interactions. This reagent is essential for various applications such as protein purification, DNA and RNA isolation, and protein refolding.
How does Guanidine Hydrochloride help in protein purification?
Guanidine Hydrochloride plays a crucial role in protein purification by denaturing the proteins, disrupting their native conformation, and promoting solubilization. This chaotropic agent helps to unfold the proteins, exposing the hydrophobic regions, which facilitates the removal of contaminants and allows for the isolation of the target protein.
Can Guanidine Hydrochloride be used for DNA and RNA isolation?
Yes, Guanidine Hydrochloride is commonly used for DNA and RNA isolation due to its ability to denature macromolecules and inhibit nuclease activity. By disrupting the structure of nucleic acids, Guanidine Hydrochloride helps to release them from cellular components and maintain their stability during extraction procedures.
How can Guanidine Hydrochloride aid in protein refolding?
Protein refolding is a challenging process that requires denaturation and renaturation of the protein to achieve its native conformation. Guanidine Hydrochloride is often used in protein refolding protocols as a denaturant to unfold misfolded proteins and facilitate their correct refolding into the biologically active form.
What are some best practices for using Guanidine Hydrochloride in molecular biology experiments?
When working with Guanidine Hydrochloride, it is important to handle the reagent with caution as it can be irritating to the skin, eyes, and respiratory tract. Use appropriate personal protective equipment such as gloves and goggles. Additionally, always prepare solutions of Guanidine Hydrochloride in a well-ventilated area and avoid inhaling the vapors. Store the reagent at the recommended temperature and protect it from light to maintain its stability for optimal performance in molecular biology applications.
