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| Name | Guanidine Thiocyanate 6 mol/L (6 M in 0.1 M Tris; pH 7.5) |
| Concentration | 6 mol/L |
| Buffer | 0.1 M Tris |
| PH | 7.5 |
| Application | Molecular biology |
| Storage | Room temperature |
| Solubility | Soluble in water |
| Appearance | Clear colorless solution |
| Stability | Stable for long-term storage |
| Usage | Commonly used for nucleic acid purification |
| Hazards | Harmful if swallowed or inhaled, causes skin and eye irritation |
| Handling | Use in a well-ventilated area, wear protective gloves and eyewear |
| Shelf Life | 1 year |
| Shipping Information | Shipped at room temperature |
FAQ
What is Guanidine Thiocyanate and how is it used in molecular biology?
Guanidine Thiocyanate is a chaotropic agent commonly used in molecular biology for various applications such as RNA and DNA extraction. It disrupts hydrogen bonding and denatures proteins, allowing for the isolation of nucleic acids from biological samples.
What is the concentration of Guanidine Thiocyanate in the provided solution?
The provided solution contains Guanidine Thiocyanate at a concentration of 6 mol/L, which is equivalent to 6 M in 0.1 M Tris buffer at pH 7.5. This concentration is commonly used for nucleic acid extraction and purification procedures.
How should Guanidine Thiocyanate be stored and handled?
Guanidine Thiocyanate should be stored in a cool, dry place away from direct sunlight and moisture. It is important to handle the chemical with care, following proper safety protocols such as wearing gloves and eye protection. In case of contact with skin or eyes, rinse immediately with plenty of water.
What are the benefits of using Guanidine Thiocyanate in molecular biology applications?
Guanidine Thiocyanate is a powerful chaotropic agent that helps in the denaturation of proteins and disruption of cellular structures, making it ideal for nucleic acid extraction. It also helps in the solubilization of proteins and RNA, aiding in downstream applications such as RT-PCR and sequencing.
Can Guanidine Thiocyanate be used in combination with other reagents for nucleic acid extraction?
Yes, Guanidine Thiocyanate can be used in combination with other reagents such as phenol-chloroform for nucleic acid extraction. It is commonly used in protocols like TRIzol extraction for RNA isolation and ChIP assays for chromatin immunoprecipitation.
Guanidine Thiocyanate is a chaotropic agent commonly used in molecular biology for various applications such as RNA and DNA extraction. It disrupts hydrogen bonding and denatures proteins, allowing for the isolation of nucleic acids from biological samples.
What is the concentration of Guanidine Thiocyanate in the provided solution?
The provided solution contains Guanidine Thiocyanate at a concentration of 6 mol/L, which is equivalent to 6 M in 0.1 M Tris buffer at pH 7.5. This concentration is commonly used for nucleic acid extraction and purification procedures.
How should Guanidine Thiocyanate be stored and handled?
Guanidine Thiocyanate should be stored in a cool, dry place away from direct sunlight and moisture. It is important to handle the chemical with care, following proper safety protocols such as wearing gloves and eye protection. In case of contact with skin or eyes, rinse immediately with plenty of water.
What are the benefits of using Guanidine Thiocyanate in molecular biology applications?
Guanidine Thiocyanate is a powerful chaotropic agent that helps in the denaturation of proteins and disruption of cellular structures, making it ideal for nucleic acid extraction. It also helps in the solubilization of proteins and RNA, aiding in downstream applications such as RT-PCR and sequencing.
Can Guanidine Thiocyanate be used in combination with other reagents for nucleic acid extraction?
Yes, Guanidine Thiocyanate can be used in combination with other reagents such as phenol-chloroform for nucleic acid extraction. It is commonly used in protocols like TRIzol extraction for RNA isolation and ChIP assays for chromatin immunoprecipitation.
