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| Name | Guanidine Thiocyanate |
| Product Type | Reagent |
| Used For | Molecular biology |
| Chemical Formula | CH5N3SCN |
| Molecular Weight | 118.16 g/mol |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| Purity | ≥ 99% |
| Storage Condition | Store at room temperature |
| Shelf Life | 2 years |
| Specificity | Highly specific for RNA isolation |
| Applications | Used for RNA extraction and purification |
| Manufacturer | Various manufacturers |
| CAS Number | 593-84-0 |
| Hazards | Irritant, Harmful if swallowed or inhaled |
FAQ
What is Guanidine Thiocyanate and how is it used in molecular biology?
Guanidine Thiocyanate is a chemical compound commonly used in molecular biology for various applications. It is particularly known for its ability to inactivate enzymes and denature proteins, making it a key reagent in processes like RNA isolation and purification. By disrupting the structure of proteins and enzymes, Guanidine Thiocyanate helps researchers extract pure nucleic acids like RNA for further analysis and experimentation.
What are the advantages of using Guanidine Thiocyanate over other methods for RNA isolation?
One of the main advantages of using Guanidine Thiocyanate for RNA isolation is its efficiency in denaturing proteins and inactivating enzymes. This helps ensure that the RNA obtained is of high purity, free from contaminants that could interfere with downstream applications like reverse transcription or PCR. Additionally, Guanidine Thiocyanate is a versatile reagent that can be easily integrated into various RNA isolation protocols, providing researchers with a reliable and consistent method for obtaining high-quality RNA.
How does Guanidine Thiocyanate facilitate RNA extraction from complex samples?
Guanidine Thiocyanate plays a crucial role in RNA extraction from complex samples by disrupting the structure of proteins and enzymes that could otherwise degrade RNA. In samples with high levels of RNases or other contaminants, Guanidine Thiocyanate helps protect the integrity of RNA during extraction, ensuring that the final product is suitable for downstream applications. By effectively inactivating enzymes and denaturing proteins, Guanidine Thiocyanate allows researchers to isolate RNA from a wide range of sample types, including tissues, blood, and cultured cells.
Can Guanidine Thiocyanate be used for other molecular biology applications besides RNA isolation?
While Guanidine Thiocyanate is commonly used for RNA isolation, it can also be employed in other molecular biology applications. For example, Guanidine Thiocyanate is often used in nucleic acid extraction and purification protocols involving DNA. Its ability to denature proteins and inactivate enzymes makes it a valuable reagent for isolating high-quality DNA from various sample types. Additionally, Guanidine Thiocyanate can be used in protein purification procedures and is a key component in certain DNA sequencing methodologies.
Are there any specific considerations to keep in mind when working with Guanidine Thiocyanate in the lab?
When working with Guanidine Thiocyanate in the lab, it is important to follow proper safety precautions to minimize the risk of exposure. Guanidine Thiocyanate should be handled in a well-ventilated area, and personal protective equipment like gloves, goggles, and lab coats should be worn at all times. Additionally, Guanidine Thiocyanate should be stored in a cool, dry place away from direct sunlight and incompatible substances. It is also important to carefully follow the manufacturer's instructions for handling and disposal to ensure the safe use of Guanidine Thiocyanate in the laboratory.
Guanidine Thiocyanate is a chemical compound commonly used in molecular biology for various applications. It is particularly known for its ability to inactivate enzymes and denature proteins, making it a key reagent in processes like RNA isolation and purification. By disrupting the structure of proteins and enzymes, Guanidine Thiocyanate helps researchers extract pure nucleic acids like RNA for further analysis and experimentation.
What are the advantages of using Guanidine Thiocyanate over other methods for RNA isolation?
One of the main advantages of using Guanidine Thiocyanate for RNA isolation is its efficiency in denaturing proteins and inactivating enzymes. This helps ensure that the RNA obtained is of high purity, free from contaminants that could interfere with downstream applications like reverse transcription or PCR. Additionally, Guanidine Thiocyanate is a versatile reagent that can be easily integrated into various RNA isolation protocols, providing researchers with a reliable and consistent method for obtaining high-quality RNA.
How does Guanidine Thiocyanate facilitate RNA extraction from complex samples?
Guanidine Thiocyanate plays a crucial role in RNA extraction from complex samples by disrupting the structure of proteins and enzymes that could otherwise degrade RNA. In samples with high levels of RNases or other contaminants, Guanidine Thiocyanate helps protect the integrity of RNA during extraction, ensuring that the final product is suitable for downstream applications. By effectively inactivating enzymes and denaturing proteins, Guanidine Thiocyanate allows researchers to isolate RNA from a wide range of sample types, including tissues, blood, and cultured cells.
Can Guanidine Thiocyanate be used for other molecular biology applications besides RNA isolation?
While Guanidine Thiocyanate is commonly used for RNA isolation, it can also be employed in other molecular biology applications. For example, Guanidine Thiocyanate is often used in nucleic acid extraction and purification protocols involving DNA. Its ability to denature proteins and inactivate enzymes makes it a valuable reagent for isolating high-quality DNA from various sample types. Additionally, Guanidine Thiocyanate can be used in protein purification procedures and is a key component in certain DNA sequencing methodologies.
Are there any specific considerations to keep in mind when working with Guanidine Thiocyanate in the lab?
When working with Guanidine Thiocyanate in the lab, it is important to follow proper safety precautions to minimize the risk of exposure. Guanidine Thiocyanate should be handled in a well-ventilated area, and personal protective equipment like gloves, goggles, and lab coats should be worn at all times. Additionally, Guanidine Thiocyanate should be stored in a cool, dry place away from direct sunlight and incompatible substances. It is also important to carefully follow the manufacturer's instructions for handling and disposal to ensure the safe use of Guanidine Thiocyanate in the laboratory.
