Back
| Name | HEPES buffer pH 8.0 (1 M) sterile |
| PH | 8 |
| Concentration | 1 M |
| Sterility | sterile |
| Volume | 1 L |
| Storage Conditions | Store at room temperature |
| Usage | Commonly used in cell culture and molecular biology |
| Composition | HEPES, water |
| Purity | High purity with low levels of contaminants |
| Stability | Stable for long term storage |
| Solubility | Highly soluble in water |
| Compatibility | Compatible with most biological applications |
| Packaging | Comes in a convenient bottle for easy dispensing |
| Manufacturer | Various manufacturers available |
| Availability | Widely available in scientific research supply stores |
| Applications | Used as a buffering agent in cell culture media and biological assays |
FAQ
What is HEPES buffer pH 8.0 (1 M) sterile and why is it important in biological
research?
HEPES buffer is a commonly used buffering agent in biological research to maintain a stable pH level in cell culture media, protein solutions, and other biological samples. HEPES buffer pH 8.0 (1 M) is specifically formulated to provide optimal buffering capacity at a pH of 8.0, which is suitable for a wide range of biological applications.
How is HEPES buffer pH 8.0 (1 M) sterile different from non-sterile buffers?
HEPES buffer pH 8.0 (1 M) sterile is specially treated to ensure that it is free from bacteria, fungi, and other contaminants that could potentially interfere with biological experiments. This is crucial for maintaining the integrity of cell cultures, protein samples, and other sensitive biological materials.
How should I store HEPES buffer pH 8.0 (1 M) sterile to ensure its stability and effectiveness?
HEPES buffer pH 8.0 (1 M) sterile should be stored at room temperature in a cool, dry place away from direct sunlight. It is important to avoid repeated freeze-thaw cycles, as this can compromise the pH and buffering capacity of the solution. Additionally, proper handling techniques should be followed to prevent contamination.
Can I use HEPES buffer pH 8.0 (1 M) sterile in a wide range of biological applications?
Yes, HEPES buffer pH 8.0 (1 M) sterile is versatile and can be used in various biological applications, including cell culture, protein biochemistry, and molecular biology. Its stable pH and high buffering capacity make it an ideal choice for maintaining optimal conditions for biological experiments.
Is HEPES buffer pH 8.0 (1 M) sterile compatible with other buffers and reagents commonly used in biological research?
HEPES buffer pH 8.0 (1 M) sterile is compatible with most common buffers and reagents used in biological research. However, it is always recommended to perform compatibility tests when combining different solutions to ensure that they do not interfere with each other or compromise the integrity of the experiment.
HEPES buffer is a commonly used buffering agent in biological research to maintain a stable pH level in cell culture media, protein solutions, and other biological samples. HEPES buffer pH 8.0 (1 M) is specifically formulated to provide optimal buffering capacity at a pH of 8.0, which is suitable for a wide range of biological applications.
How is HEPES buffer pH 8.0 (1 M) sterile different from non-sterile buffers?
HEPES buffer pH 8.0 (1 M) sterile is specially treated to ensure that it is free from bacteria, fungi, and other contaminants that could potentially interfere with biological experiments. This is crucial for maintaining the integrity of cell cultures, protein samples, and other sensitive biological materials.
How should I store HEPES buffer pH 8.0 (1 M) sterile to ensure its stability and effectiveness?
HEPES buffer pH 8.0 (1 M) sterile should be stored at room temperature in a cool, dry place away from direct sunlight. It is important to avoid repeated freeze-thaw cycles, as this can compromise the pH and buffering capacity of the solution. Additionally, proper handling techniques should be followed to prevent contamination.
Can I use HEPES buffer pH 8.0 (1 M) sterile in a wide range of biological applications?
Yes, HEPES buffer pH 8.0 (1 M) sterile is versatile and can be used in various biological applications, including cell culture, protein biochemistry, and molecular biology. Its stable pH and high buffering capacity make it an ideal choice for maintaining optimal conditions for biological experiments.
Is HEPES buffer pH 8.0 (1 M) sterile compatible with other buffers and reagents commonly used in biological research?
HEPES buffer pH 8.0 (1 M) sterile is compatible with most common buffers and reagents used in biological research. However, it is always recommended to perform compatibility tests when combining different solutions to ensure that they do not interfere with each other or compromise the integrity of the experiment.
