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| Product | HEPES for buffer solutions |
| Appearance | White crystalline powder |
| Molecular Formula | C8H18N2O4S |
| Molecular Weight | 238.3 g/mol |
| CAS Number | 7365-45-9 |
| Solubility | Soluble in water |
| Storage Conditions | Store at room temperature |
| Purity | ≥ 99% |
| Uses | Buffering agent in biological research |
| Shelf Life | 2 years |
| Supplier | Various chemical suppliers |
FAQ
What is HEPES and why is it used for buffer solutions?
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a biological buffer commonly used in cell culture and molecular biology applications. It helps to maintain a stable pH in buffer solutions, making it ideal for maintaining the correct conditions for biochemical reactions and cell culture.
How does HEPES maintain pH stability in buffer solutions?
HEPES acts as a buffer by accepting or donating protons to maintain a stable pH in a solution. Its pKa value of around 7.55 makes it effective at buffering in the physiological pH range, making it a popular choice for biological applications.
What are some advantages of using HEPES for buffer solutions?
One of the main advantages of using HEPES is its ability to maintain a stable pH over a wide range of temperatures and concentrations. It is also non-toxic to cells, making it suitable for cell culture applications. Additionally, HEPES has a high buffering capacity, making it effective at maintaining pH in various experimental conditions.
Can HEPES be used in combination with other buffers?
HEPES can be used in combination with other buffers to achieve specific pH requirements. It is often used in conjunction with other buffering agents such as MOPS or Tris to create custom buffer solutions tailored to the needs of the experiment. Care should be taken to ensure that the combined buffers do not interfere with each other's buffering capacities.
What is the recommended concentration of HEPES for buffer solutions?
The recommended concentration of HEPES for buffer solutions typically ranges from 5mM to 50mM, depending on the specific requirements of the experiment. It is important to optimize the concentration of HEPES based on factors such as pH stability, buffering capacity, and cell compatibility.
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a biological buffer commonly used in cell culture and molecular biology applications. It helps to maintain a stable pH in buffer solutions, making it ideal for maintaining the correct conditions for biochemical reactions and cell culture.
How does HEPES maintain pH stability in buffer solutions?
HEPES acts as a buffer by accepting or donating protons to maintain a stable pH in a solution. Its pKa value of around 7.55 makes it effective at buffering in the physiological pH range, making it a popular choice for biological applications.
What are some advantages of using HEPES for buffer solutions?
One of the main advantages of using HEPES is its ability to maintain a stable pH over a wide range of temperatures and concentrations. It is also non-toxic to cells, making it suitable for cell culture applications. Additionally, HEPES has a high buffering capacity, making it effective at maintaining pH in various experimental conditions.
Can HEPES be used in combination with other buffers?
HEPES can be used in combination with other buffers to achieve specific pH requirements. It is often used in conjunction with other buffering agents such as MOPS or Tris to create custom buffer solutions tailored to the needs of the experiment. Care should be taken to ensure that the combined buffers do not interfere with each other's buffering capacities.
What is the recommended concentration of HEPES for buffer solutions?
The recommended concentration of HEPES for buffer solutions typically ranges from 5mM to 50mM, depending on the specific requirements of the experiment. It is important to optimize the concentration of HEPES based on factors such as pH stability, buffering capacity, and cell compatibility.
