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| Name | HEPES for cell culture |
| Type | Buffer solution |
| Size | 500mL |
| Concentration | 1M |
| PH | 7.2-7.5 |
| Storage | Store at room temperature |
| Sterility | Sterile filtered |
| Composition | HEPES (25mM), NaCl (135mM), KCl (5mM), CaCl2 (2mM), MgCl2 (1mM), D-Glucose (10mM) |
| Applications | Maintains pH in cell culture media, acts as a buffering agent |
| Compatibility | Compatible with a wide range of cell types |
| Shelf Life | 2 years |
| Manufacturer | Various manufacturers |
FAQ
What is HEPES and how is it used in cell culture?
HEPES, or N-2-Hydroxyethylpiperazine-N’-2-ethanesulfonic acid, is a commonly used buffer in cell culture media. It helps maintain a stable pH in culture medium, which is essential for the survival and growth of cells. HEPES is especially useful for cell culture systems that require higher buffering capacity and minimal shifts in pH.
Why is HEPES preferred over other buffers in cell culture?
HEPES is preferred in cell culture for several reasons. First, it has a high buffering capacity, which means it can effectively maintain a stable pH in the medium. Additionally, HEPES is zwitterionic, meaning it carries both positive and negative charges, allowing it to buffer effectively in a wide range of physiological conditions. HEPES is also chemically stable and non-toxic to cells, making it an ideal choice for long-term cell culture experiments.
How do I use HEPES in my cell culture experiments?
HEPES is typically added to cell culture media at a concentration of 10-25mM. It is important to dissolve HEPES completely in the medium before adjusting the pH to the desired level. HEPES is typically used in conjunction with CO2 bicarbonate buffering systems to maintain a stable pH in the culture environment. It is important to note that HEPES can interfere with certain cell assays, so it is necessary to optimize its concentration for specific experimental conditions.
Are there any considerations or precautions to keep in mind when using HEPES in cell culture?
While HEPES is generally considered safe and non-toxic to cells, there are a few precautions to keep in mind. HEPES is a slightly acidic buffer, so it is important to adjust the pH of the medium accordingly when using HEPES. Additionally, HEPES can interfere with certain analytical techniques such as absorbance readings, so it is important to consider this when designing experiments. Overall, HEPES is a versatile and effective buffer for cell culture, but it is important to optimize its use for specific experimental conditions.
What are the advantages of using HEPES in cell culture over other buffers?
HEPES offers several advantages over other buffers in cell culture. Its high buffering capacity and ability to maintain a stable pH make it ideal for long-term cell culture experiments. HEPES is also compatible with a wide range of cell lines and is non-toxic to cells, making it a versatile choice for cell culture applications. Additionally, HEPES is chemically stable, which means it can be used in a variety of experimental conditions without degradation. Overall, HEPES is a reliable and effective buffer for cell culture that provides consistent pH control and optimal cell growth.
HEPES, or N-2-Hydroxyethylpiperazine-N’-2-ethanesulfonic acid, is a commonly used buffer in cell culture media. It helps maintain a stable pH in culture medium, which is essential for the survival and growth of cells. HEPES is especially useful for cell culture systems that require higher buffering capacity and minimal shifts in pH.
Why is HEPES preferred over other buffers in cell culture?
HEPES is preferred in cell culture for several reasons. First, it has a high buffering capacity, which means it can effectively maintain a stable pH in the medium. Additionally, HEPES is zwitterionic, meaning it carries both positive and negative charges, allowing it to buffer effectively in a wide range of physiological conditions. HEPES is also chemically stable and non-toxic to cells, making it an ideal choice for long-term cell culture experiments.
How do I use HEPES in my cell culture experiments?
HEPES is typically added to cell culture media at a concentration of 10-25mM. It is important to dissolve HEPES completely in the medium before adjusting the pH to the desired level. HEPES is typically used in conjunction with CO2 bicarbonate buffering systems to maintain a stable pH in the culture environment. It is important to note that HEPES can interfere with certain cell assays, so it is necessary to optimize its concentration for specific experimental conditions.
Are there any considerations or precautions to keep in mind when using HEPES in cell culture?
While HEPES is generally considered safe and non-toxic to cells, there are a few precautions to keep in mind. HEPES is a slightly acidic buffer, so it is important to adjust the pH of the medium accordingly when using HEPES. Additionally, HEPES can interfere with certain analytical techniques such as absorbance readings, so it is important to consider this when designing experiments. Overall, HEPES is a versatile and effective buffer for cell culture, but it is important to optimize its use for specific experimental conditions.
What are the advantages of using HEPES in cell culture over other buffers?
HEPES offers several advantages over other buffers in cell culture. Its high buffering capacity and ability to maintain a stable pH make it ideal for long-term cell culture experiments. HEPES is also compatible with a wide range of cell lines and is non-toxic to cells, making it a versatile choice for cell culture applications. Additionally, HEPES is chemically stable, which means it can be used in a variety of experimental conditions without degradation. Overall, HEPES is a reliable and effective buffer for cell culture that provides consistent pH control and optimal cell growth.
