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| Product Name | HEPES for molecular biology |
| Product Type | Buffer solution |
| Package Size | 100 mL |
| Concentration | 1 M |
| PH Range | 7.2 - 7.6 |
| Storage Temperature | Room temperature |
| Shelf Life | 2 years |
| Sterility | Non-sterile |
| Applications | Cell culture, molecular biology |
| Compatibility | Compatible with most cell types and molecular biology applications |
| Manufacturing Process | Produced under strict quality control standards |
| Supplied Form | Liquid |
| Purity | High purity |
| Shipping Restrictions | May require expedited shipping |
| Storage Instructions | Store in a cool, dry place |
| Handling Precautions | Use appropriate personal protective equipment |
FAQ
What is HEPES and how is it used in molecular biology?
HEPES, or N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, is a common buffer used in molecular biology and biochemistry. It is particularly useful in cell culture and protein studies due to its ability to maintain a stable pH range between 6.8 and 8.2. HEPES is often used as a buffer in cell culture media, especially when working with sensitive cells that require a stable pH environment.
How does HEPES differ from other buffers commonly used in molecular biology?
HEPES offers several advantages over other buffers commonly used in molecular biology. Unlike phosphate buffers, HEPES does not interfere with enzyme activity, making it ideal for enzyme assays and other biochemical studies. Additionally, HEPES has a buffering capacity that is relatively unaffected by changes in temperature, which makes it particularly useful for experiments that require precise control over pH.
Can HEPES be used in a wide range of experimental conditions?
Yes, HEPES is extremely versatile and can be used in a wide range of experimental conditions. It is commonly used in cell culture media, protein purification, enzyme assays, and other applications where a stable pH range is required. HEPES is compatible with a variety of cell types and has been shown to support the growth of both adherent and suspension cells.
How should HEPES be stored to ensure its effectiveness?
HEPES should be stored in a dry, cool place away from direct sunlight. It is important to keep the container tightly sealed to prevent contamination and degradation of the buffer. Additionally, HEPES should be protected from moisture, as exposure to water can affect its pH buffering capacity. When stored properly, HEPES has a long shelf life and can be used for multiple experiments.
Are there any potential limitations or considerations when using HEPES in molecular biology experiments?
While HEPES is a highly effective buffer for many applications in molecular biology, there are some limitations to consider. HEPES is not recommended for experiments that require extremely low or high pH ranges, as its buffering capacity is optimized for pH levels between 6.8 and 8.2. Additionally, HEPES is not suitable for use in experiments that involve heavy metal ions, as it can form complexes with these ions that may interfere with experimental results. Overall, HEPES is a versatile and reliable buffer that is widely used in molecular biology research.
HEPES, or N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, is a common buffer used in molecular biology and biochemistry. It is particularly useful in cell culture and protein studies due to its ability to maintain a stable pH range between 6.8 and 8.2. HEPES is often used as a buffer in cell culture media, especially when working with sensitive cells that require a stable pH environment.
How does HEPES differ from other buffers commonly used in molecular biology?
HEPES offers several advantages over other buffers commonly used in molecular biology. Unlike phosphate buffers, HEPES does not interfere with enzyme activity, making it ideal for enzyme assays and other biochemical studies. Additionally, HEPES has a buffering capacity that is relatively unaffected by changes in temperature, which makes it particularly useful for experiments that require precise control over pH.
Can HEPES be used in a wide range of experimental conditions?
Yes, HEPES is extremely versatile and can be used in a wide range of experimental conditions. It is commonly used in cell culture media, protein purification, enzyme assays, and other applications where a stable pH range is required. HEPES is compatible with a variety of cell types and has been shown to support the growth of both adherent and suspension cells.
How should HEPES be stored to ensure its effectiveness?
HEPES should be stored in a dry, cool place away from direct sunlight. It is important to keep the container tightly sealed to prevent contamination and degradation of the buffer. Additionally, HEPES should be protected from moisture, as exposure to water can affect its pH buffering capacity. When stored properly, HEPES has a long shelf life and can be used for multiple experiments.
Are there any potential limitations or considerations when using HEPES in molecular biology experiments?
While HEPES is a highly effective buffer for many applications in molecular biology, there are some limitations to consider. HEPES is not recommended for experiments that require extremely low or high pH ranges, as its buffering capacity is optimized for pH levels between 6.8 and 8.2. Additionally, HEPES is not suitable for use in experiments that involve heavy metal ions, as it can form complexes with these ions that may interfere with experimental results. Overall, HEPES is a versatile and reliable buffer that is widely used in molecular biology research.
