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| Product Name | HEPPS for buffer solutions |
| Supplier | Sigma-Aldrich |
| Chemical Formula | C18H28N2O4S |
| Molecular Weight | 360.5 g/mol |
| PH Range | 6.8-8.2 |
| Solubility | Soluble in water |
| Storage Conditions | Store at room temperature |
| Shelf Life | Minimum of 2 years |
| Appearance | White to off-white crystalline powder |
| Usage | Commonly used as a buffer in biochemical and molecular biological research |
| Applications | Cell culture, protein purification, enzyme assays |
| Packaging Options | Available in various quantities including 25g, 100g, and 500g |
| Alternative Names | EPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) |
FAQ
What is HEPPS and how is it used in buffer solutions?
HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a buffering agent commonly used in biological and biochemical research. It is a zwitterionic compound that helps maintain a stable pH level in solutions by resisting changes in pH when acids or bases are added.
What are the advantages of using HEPPS in buffer solutions?
HEPPS offers several advantages as a buffering agent. It has a strong buffering capacity in the pH range of 6.8-8.2, making it ideal for many biological and biochemical applications. HEPPS is also water-soluble and readily available in high purity, ensuring accurate and reliable results in experiments.
How do I prepare a buffer solution using HEPPS?
To prepare a buffer solution using HEPPS, first determine the desired pH of the solution. Calculate the amount of HEPPS needed to achieve the desired pH using a buffer calculator or reference table. Dissolve the calculated amount of HEPPS in water, adjusting the pH with either hydrochloric acid (HCl) or sodium hydroxide (NaOH) as needed. Finally, dilute the solution to the desired volume with water.
Can HEPPS be used in combination with other buffering agents?
Yes, HEPPS can be used in combination with other buffering agents to create custom buffer solutions with specific pH ranges and buffering capacities. By combining HEPPS with other compounds such as MES or Tris, researchers can tailor buffer solutions to meet the requirements of their experiments.
Are there any special storage or handling considerations for HEPPS?
HEPPS should be stored in a cool, dry place away from heat and light to prevent degradation. When handling HEPPS, use appropriate personal protective equipment such as gloves and goggles to avoid skin and eye contact. It is also important to properly label all solutions containing HEPPS to prevent confusion and ensure safety in the laboratory.
HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a buffering agent commonly used in biological and biochemical research. It is a zwitterionic compound that helps maintain a stable pH level in solutions by resisting changes in pH when acids or bases are added.
What are the advantages of using HEPPS in buffer solutions?
HEPPS offers several advantages as a buffering agent. It has a strong buffering capacity in the pH range of 6.8-8.2, making it ideal for many biological and biochemical applications. HEPPS is also water-soluble and readily available in high purity, ensuring accurate and reliable results in experiments.
How do I prepare a buffer solution using HEPPS?
To prepare a buffer solution using HEPPS, first determine the desired pH of the solution. Calculate the amount of HEPPS needed to achieve the desired pH using a buffer calculator or reference table. Dissolve the calculated amount of HEPPS in water, adjusting the pH with either hydrochloric acid (HCl) or sodium hydroxide (NaOH) as needed. Finally, dilute the solution to the desired volume with water.
Can HEPPS be used in combination with other buffering agents?
Yes, HEPPS can be used in combination with other buffering agents to create custom buffer solutions with specific pH ranges and buffering capacities. By combining HEPPS with other compounds such as MES or Tris, researchers can tailor buffer solutions to meet the requirements of their experiments.
Are there any special storage or handling considerations for HEPPS?
HEPPS should be stored in a cool, dry place away from heat and light to prevent degradation. When handling HEPPS, use appropriate personal protective equipment such as gloves and goggles to avoid skin and eye contact. It is also important to properly label all solutions containing HEPPS to prevent confusion and ensure safety in the laboratory.
