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| Name | MOPS for molecular biology |
| Brand | Thermo Fisher Scientific |
| Product Type | Buffer |
| Size | 500 mL |
| PH Range | 6.5 - 7.9 |
| Concentration | 1X |
| Storage Conditions | Store at room temperature |
| Used For | Molecular biology experiments |
| Composition | 3-(N-morpholino)propanesulfonic acid, sodium hydroxide, water |
| Shelf Life | 2 years |
| Sterilization Method | Filtration |
| Formulation | Ready-to-use liquid |
| Quality Control Methods | QC tested to ensure consistent performance |
| Applications | Polymerase chain reaction (PCR), DNA gel electrophoresis, protein electrophoresis |
| Compatibility | Compatible with common molecular biology techniques and reagents |
| Manufacturing Process | Manufactured under ISO 9001:2015 and ISO 13485:2016 quality standards |
| Shipping Restrictions | Ships at ambient temperature |
FAQ
What is MOPS and how is it used in molecular biology?
MOPS, or 3-(N-Morpholino)propanesulfonic acid, is a buffering agent commonly used in molecular biology research. It helps to maintain a stable pH level in solutions, which is crucial for many enzymatic reactions and other biological processes. In molecular biology, MOPS is often used in electrophoresis buffers and cell culture media.
What are the benefits of using MOPS in molecular biology experiments?
One of the main benefits of using MOPS is its ability to maintain a consistent pH level over a wide range of temperatures. This is particularly useful in experiments that involve heating or cooling samples, as fluctuations in pH can alter the results. Additionally, MOPS is known for its low toxicity, making it a safe and reliable option for researchers working with biological samples.
How can MOPS be used in electrophoresis applications?
In electrophoresis applications, MOPS is commonly used as a buffering agent in running buffers. It helps to maintain a stable pH throughout the electrophoresis process, which is essential for separating DNA, RNA, or proteins based on their size and charge. MOPS is also compatible with a variety of gel types, making it a versatile option for different types of electrophoresis experiments.
What makes MOPS a preferred choice for cell culture media in molecular biology research?
MOPS is often used in cell culture media due to its ability to maintain a stable pH in the presence of CO2, which is critical for cell growth and viability. The buffering capacity of MOPS helps to regulate the pH of the media, ensuring optimal conditions for cell culture. Additionally, MOPS has low cytotoxicity, making it a safe choice for maintaining healthy cells in culture.
Are there any specific considerations to keep in mind when using MOPS in molecular biology experiments?
When using MOPS in molecular biology experiments, it is important to accurately prepare and measure the solution to achieve the desired pH level. It is also recommended to store MOPS powder in a cool, dry place to prevent contamination or degradation. Additionally, researchers should be aware of the potential interactions between MOPS and other components in their experiments, such as enzymes or nucleic acids, to ensure accurate and reliable results.
MOPS, or 3-(N-Morpholino)propanesulfonic acid, is a buffering agent commonly used in molecular biology research. It helps to maintain a stable pH level in solutions, which is crucial for many enzymatic reactions and other biological processes. In molecular biology, MOPS is often used in electrophoresis buffers and cell culture media.
What are the benefits of using MOPS in molecular biology experiments?
One of the main benefits of using MOPS is its ability to maintain a consistent pH level over a wide range of temperatures. This is particularly useful in experiments that involve heating or cooling samples, as fluctuations in pH can alter the results. Additionally, MOPS is known for its low toxicity, making it a safe and reliable option for researchers working with biological samples.
How can MOPS be used in electrophoresis applications?
In electrophoresis applications, MOPS is commonly used as a buffering agent in running buffers. It helps to maintain a stable pH throughout the electrophoresis process, which is essential for separating DNA, RNA, or proteins based on their size and charge. MOPS is also compatible with a variety of gel types, making it a versatile option for different types of electrophoresis experiments.
What makes MOPS a preferred choice for cell culture media in molecular biology research?
MOPS is often used in cell culture media due to its ability to maintain a stable pH in the presence of CO2, which is critical for cell growth and viability. The buffering capacity of MOPS helps to regulate the pH of the media, ensuring optimal conditions for cell culture. Additionally, MOPS has low cytotoxicity, making it a safe choice for maintaining healthy cells in culture.
Are there any specific considerations to keep in mind when using MOPS in molecular biology experiments?
When using MOPS in molecular biology experiments, it is important to accurately prepare and measure the solution to achieve the desired pH level. It is also recommended to store MOPS powder in a cool, dry place to prevent contamination or degradation. Additionally, researchers should be aware of the potential interactions between MOPS and other components in their experiments, such as enzymes or nucleic acids, to ensure accurate and reliable results.
