PBS buffer (1X, Dulbecco's) - Powder

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Product Name PBS buffer (1X, Dulbecco's) - Powder
Buffer Type Phosphate Buffered Saline
Concentration 1X
Form Powder
Storage Store at room temperature
PH Range 7.2 - 7.6
Sterility Non-sterile
Endotoxin Level Not specified
Applications Cell culture, molecular biology
Ingredients Sodium chloride, potassium chloride, disodium phosphate, monopotassium phosphate
Appearance White powder
Solubility Soluble in water
Shelf Life 2 years
Manufacturer Not specified
Catalog Number Not specified
Shipping Condition Ambient temperature
Country Of Origin Not specified
Hazards May cause irritation to respiratory system if inhaled
Hazards Description Not classified as hazardous according to OSHA guidelines
Quality Control Not specified
FAQ
What is PBS buffer (1X, Dulbecco's) powder?

PBS buffer (1X, Dulbecco's) powder is a convenient and versatile solution for preparing phosphate-buffered saline (PBS) for cell culture and various molecular biology applications. This powder is formulated to dissolve easily in water, providing a standardized 1X concentration of PBS for your experiments.

How is PBS buffer (1X, Dulbecco's) powder used in research?

PBS buffer (1X, Dulbecco's) powder is commonly used in cell culture, protein purification, immunoprecipitation, and various other molecular biology techniques. It helps maintain a stable pH and osmolarity for maintaining cell viability and function during experiments. Additionally, PBS buffer can be used for washing and diluting cells, tissues, and proteins.

What are the advantages of using PBS buffer (1X, Dulbecco's) powder?

One of the main advantages of using PBS buffer (1X, Dulbecco's) powder is its convenience and ease of use. By simply dissolving the powder in water, researchers can quickly prepare a standardized buffer solution without the need for weighing out individual components. Furthermore, PBS buffer is compatible with a wide range of cell types and is gentle enough to use in various biological assays.

How should PBS buffer (1X, Dulbecco's) powder be stored?

PBS buffer (1X, Dulbecco's) powder should be stored in a cool, dry place away from direct sunlight and moisture. It is recommended to store the powder at room temperature to ensure its stability and performance. Once reconstituted, the prepared PBS buffer should be stored at 4°C and used within a few weeks for optimal results.

Can PBS buffer (1X, Dulbecco's) powder be used in combination with other reagents or buffers?

Yes, PBS buffer (1X, Dulbecco's) powder can be combined with other reagents or buffers to customize the composition based on specific experimental needs. Researchers often supplement PBS buffer with calcium, magnesium, or other additives to create specialized buffer solutions for their applications. It is important to validate the compatibility of additional components with PBS buffer to ensure the desired results.
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