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| Name | PIPES for buffer solutions |
| Brand | Sigma-Aldrich |
| Application | Buffering agent for biological and biochemical research |
| Chemical Formula | C8H18N2O6S2 |
| Molecular Weight | 302.37 g/mol |
| Solubility | Soluble in water |
| PKa | 6.76 (25°C) |
| Appearance | White crystalline powder |
| Storage Condition | Store at room temperature |
| CAS Number | 5625-37-6 |
| Purity | ≥99% |
| Packaging | Available in various pack sizes |
FAQ
What are PIPES buffer solutions and why are they important in research?
PIPED buffer solutions are chemicals commonly used in biological and biochemical research to create a stable pH environment. They are essential for maintaining the correct pH levels in experiments involving biological molecules, as small changes in pH can significantly affect the outcome of the experiment.
How are PIPES buffer solutions different from other types of buffer solutions?
PIPED buffer solutions are unique in that they are particularly effective at maintaining a stable pH in the range of 6.1 to 7.5. This makes them ideal for many biological experiments that require a slightly acidic to neutral pH range. Additionally, PIPED buffer solutions have low UV absorbance, making them suitable for experiments involving UV detection.
What are some common applications of PIPES buffer solutions in research?
PIPED buffer solutions are commonly used in a variety of biological experiments, including enzyme assays, protein purification, and cell culture work. They are also frequently used in molecular biology techniques such as PCR and DNA sequencing.
How should PIPES buffer solutions be prepared and stored for optimal performance?
To prepare PIPED buffer solutions, simply dissolve the appropriate amount of powder in distilled water and adjust the pH as needed. Once prepared, PIPES buffer solutions should be stored at the recommended temperature and pH according to the manufacturer's instructions to ensure their stability and effectiveness.
Are there any potential risks or precautions to consider when using PIPES buffer solutions in the laboratory?
While PIPED buffer solutions are generally considered safe to use in the laboratory, it is important to take proper precautions when handling any chemicals. Always wear appropriate personal protective equipment, follow good laboratory practices, and consult the safety data sheet for specific handling instructions.
PIPED buffer solutions are chemicals commonly used in biological and biochemical research to create a stable pH environment. They are essential for maintaining the correct pH levels in experiments involving biological molecules, as small changes in pH can significantly affect the outcome of the experiment.
How are PIPES buffer solutions different from other types of buffer solutions?
PIPED buffer solutions are unique in that they are particularly effective at maintaining a stable pH in the range of 6.1 to 7.5. This makes them ideal for many biological experiments that require a slightly acidic to neutral pH range. Additionally, PIPED buffer solutions have low UV absorbance, making them suitable for experiments involving UV detection.
What are some common applications of PIPES buffer solutions in research?
PIPED buffer solutions are commonly used in a variety of biological experiments, including enzyme assays, protein purification, and cell culture work. They are also frequently used in molecular biology techniques such as PCR and DNA sequencing.
How should PIPES buffer solutions be prepared and stored for optimal performance?
To prepare PIPED buffer solutions, simply dissolve the appropriate amount of powder in distilled water and adjust the pH as needed. Once prepared, PIPES buffer solutions should be stored at the recommended temperature and pH according to the manufacturer's instructions to ensure their stability and effectiveness.
Are there any potential risks or precautions to consider when using PIPES buffer solutions in the laboratory?
While PIPED buffer solutions are generally considered safe to use in the laboratory, it is important to take proper precautions when handling any chemicals. Always wear appropriate personal protective equipment, follow good laboratory practices, and consult the safety data sheet for specific handling instructions.
