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| Product Name | TAE buffer (10X) |
| Common Use | electrophoresis of nucleic acids |
| Composition | Tris base, acetic acid, EDTA |
| PH Range | 8.0-8.3 |
| Storage Temperature | room temperature |
| Shelf Life | 2 years |
| Preparation | dilution with deionized water to desired concentration |
| Concentration | 10X |
| Compatibility | compatible with agarose gels |
| Application | DNA and RNA electrophoresis |
| Buffer Capacity | maintains stable pH during electrophoresis |
| Manufacturer | various molecular biology suppliers |
| Packaging | bottles or bags |
| Volume Options | 100 ml, 500 ml, 1 L |
| Safety Information | irritating to eyes and skin, wear appropriate PPE |
| Shipping Information | may require special handling due to chemical composition |
FAQ
What is TAE buffer (10X) and why is it important in molecular biology
experiments?
TAE buffer (Tris-Acetate-EDTA) is a commonly used buffer solution in molecular biology experiments, especially in agarose gel electrophoresis. It consists of Tris base, acetic acid, and EDTA, and is typically used in a 1X concentration for DNA and RNA electrophoresis.
What are the main functions of TAE buffer (10X) in molecular biology experiments?
TAE buffer serves several important functions in molecular biology experiments, including providing a stable pH environment for the separation of nucleic acids, facilitating the movement of DNA or RNA molecules during electrophoresis, and protecting nucleic acids from enzymatic degradation.
How is TAE buffer (10X) prepared for use in molecular biology experiments?
TAE buffer is typically prepared by diluting a concentrated 10X stock solution with distilled water to achieve the desired working concentration (usually 1X). The buffer solution is then mixed thoroughly to ensure uniformity before use in electrophoresis or other molecular biology applications.
Can TAE buffer (10X) be used interchangeably with other buffer solutions in molecular biology experiments?
While TAE buffer is commonly used in molecular biology experiments, it is not always interchangeable with other buffer solutions such as TBE (Tris-Borate-EDTA). The choice of buffer may depend on the specific requirements of the experiment, including the size of the nucleic acids being separated and the desired resolution.
What are some tips for handling and storing TAE buffer (10X) to ensure optimal performance in molecular biology experiments?
To maintain the stability and effectiveness of TAE buffer, it is important to store it properly at the recommended temperature and protect it from contamination. Additionally, proper labeling and documentation of the buffer solution can help ensure accurate and reliable results in molecular biology experiments.
TAE buffer (Tris-Acetate-EDTA) is a commonly used buffer solution in molecular biology experiments, especially in agarose gel electrophoresis. It consists of Tris base, acetic acid, and EDTA, and is typically used in a 1X concentration for DNA and RNA electrophoresis.
What are the main functions of TAE buffer (10X) in molecular biology experiments?
TAE buffer serves several important functions in molecular biology experiments, including providing a stable pH environment for the separation of nucleic acids, facilitating the movement of DNA or RNA molecules during electrophoresis, and protecting nucleic acids from enzymatic degradation.
How is TAE buffer (10X) prepared for use in molecular biology experiments?
TAE buffer is typically prepared by diluting a concentrated 10X stock solution with distilled water to achieve the desired working concentration (usually 1X). The buffer solution is then mixed thoroughly to ensure uniformity before use in electrophoresis or other molecular biology applications.
Can TAE buffer (10X) be used interchangeably with other buffer solutions in molecular biology experiments?
While TAE buffer is commonly used in molecular biology experiments, it is not always interchangeable with other buffer solutions such as TBE (Tris-Borate-EDTA). The choice of buffer may depend on the specific requirements of the experiment, including the size of the nucleic acids being separated and the desired resolution.
What are some tips for handling and storing TAE buffer (10X) to ensure optimal performance in molecular biology experiments?
To maintain the stability and effectiveness of TAE buffer, it is important to store it properly at the recommended temperature and protect it from contamination. Additionally, proper labeling and documentation of the buffer solution can help ensure accurate and reliable results in molecular biology experiments.
