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| Name | TAE buffer (50X) |
| Size | 1 L |
| Concentration | 50X |
| PH | 8.3 |
| Composition | Tris Base, Acetic Acid, EDTA |
| Storage Condition | Store at room temperature |
| Application | Electrophoresis buffer |
| Compatibility | Compatible with RNA and DNA electrophoresis |
| Purpose | Maintain pH during electrophoresis |
| Shelf Life | 2 years |
| Manufacturing Company | Thermo Fisher Scientific |
FAQ
What is TAE buffer (50X) used for?
TAE buffer (50X) is commonly used in molecular biology techniques such as agarose gel electrophoresis, DNA and RNA gel extraction, and preparation of DNA restriction digests. It provides the necessary ions and pH for optimal DNA separation and visualization.
How should TAE buffer (50X) be diluted for use in agarose gel electrophoresis?
TAE buffer (50X) should be diluted to a final concentration of 1X for use in agarose gel electrophoresis. This can be achieved by diluting the 50X stock solution with distilled water at a 1:50 ratio. For example, to prepare 1 liter of 1X TAE buffer, you would mix 20 ml of the 50X stock solution with 980 ml of distilled water.
What are the advantages of using TAE buffer (50X) over other types of electrophoresis buffers?
TAE buffer (50X) is preferred by many researchers for agarose gel electrophoresis due to its excellent buffering capacity, low conductivity, and compatibility with a wide range of DNA sizes. It also provides sharper DNA bands and better resolution compared to other commonly used electrophoresis buffers.
Can TAE buffer (50X) be used for RNA analysis as well?
Yes, TAE buffer (50X) can be used for RNA analysis in addition to DNA analysis. However, for optimal RNA visualization, it is recommended to use a denaturing agarose gel electrophoresis system with formaldehyde or glyoxal in the gel and running buffer.
How should TAE buffer (50X) be stored for long-term stability?
TAE buffer (50X) should be stored at room temperature or at 4°C for long-term stability. Avoid repeated freeze-thaw cycles and exposure to light, as this can degrade the buffer components and reduce its effectiveness. It is important to store the buffer in a tightly sealed container to prevent contamination.
TAE buffer (50X) is commonly used in molecular biology techniques such as agarose gel electrophoresis, DNA and RNA gel extraction, and preparation of DNA restriction digests. It provides the necessary ions and pH for optimal DNA separation and visualization.
How should TAE buffer (50X) be diluted for use in agarose gel electrophoresis?
TAE buffer (50X) should be diluted to a final concentration of 1X for use in agarose gel electrophoresis. This can be achieved by diluting the 50X stock solution with distilled water at a 1:50 ratio. For example, to prepare 1 liter of 1X TAE buffer, you would mix 20 ml of the 50X stock solution with 980 ml of distilled water.
What are the advantages of using TAE buffer (50X) over other types of electrophoresis buffers?
TAE buffer (50X) is preferred by many researchers for agarose gel electrophoresis due to its excellent buffering capacity, low conductivity, and compatibility with a wide range of DNA sizes. It also provides sharper DNA bands and better resolution compared to other commonly used electrophoresis buffers.
Can TAE buffer (50X) be used for RNA analysis as well?
Yes, TAE buffer (50X) can be used for RNA analysis in addition to DNA analysis. However, for optimal RNA visualization, it is recommended to use a denaturing agarose gel electrophoresis system with formaldehyde or glyoxal in the gel and running buffer.
How should TAE buffer (50X) be stored for long-term stability?
TAE buffer (50X) should be stored at room temperature or at 4°C for long-term stability. Avoid repeated freeze-thaw cycles and exposure to light, as this can degrade the buffer components and reduce its effectiveness. It is important to store the buffer in a tightly sealed container to prevent contamination.
