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| Product Name | TBE buffer (10X) |
| Brand | Not specified |
| Volume | 1 L |
| Concentration | 10X |
| Buffer Components | Tris base, Boric acid, EDTA |
| Application | Used in gel electrophoresis for nucleic acid separation |
| Storage Conditions | Store at room temperature |
| PH Range | 8.3 - 8.6 |
| Stability | Stable for at least 2 years when stored properly |
| Appearance | Clear, colorless liquid |
| Solubility | Fully soluble in water |
| Usage | Dilute to desired concentration before use |
FAQ
What is TBE buffer (10X) and what is it used for?
TBE buffer (Tris-Borate-EDTA) is a commonly used buffer in molecular biology applications, such as DNA and RNA electrophoresis. It is typically used in gel electrophoresis to separate nucleic acids based on size and charge.
How is TBE buffer (10X) different from other buffers?
TBE buffer is specifically designed for nucleic acid electrophoresis, providing a stable pH and buffering capacity that is ideal for separating DNA and RNA molecules. It is often preferred over other buffers, such as TAE buffer, for its ability to resolve small DNA fragments more effectively.
What is the concentration of TBE buffer (10X) and how is it prepared for use?
TBE buffer is typically supplied as a 10X concentrated solution, which means it needs to be diluted before use. To prepare a 1X working solution, simply dilute the 10X stock solution with distilled water at a 1:10 ratio. For example, mix 100 ml of 10X TBE buffer with 900 ml of distilled water to make 1 liter of 1X TBE buffer.
Can TBE buffer (10X) be used for other applications besides nucleic acid electrophoresis?
TBE buffer is primarily used for DNA and RNA electrophoresis, but it can also be used for other applications such as running protein gels for SDS-PAGE. However, TBE buffer is not recommended for protein electrophoresis as it may interfere with protein migration and resolution.
Are there any precautions or special storage requirements for TBE buffer (10X)?
TBE buffer should be stored at room temperature and protected from light to prevent degradation. It is also important to avoid contamination of the buffer with nucleases or other impurities that may affect the quality of electrophoresis results. Additionally, always wear appropriate personal protective equipment when handling TBE buffer to avoid skin or eye irritation.
TBE buffer (Tris-Borate-EDTA) is a commonly used buffer in molecular biology applications, such as DNA and RNA electrophoresis. It is typically used in gel electrophoresis to separate nucleic acids based on size and charge.
How is TBE buffer (10X) different from other buffers?
TBE buffer is specifically designed for nucleic acid electrophoresis, providing a stable pH and buffering capacity that is ideal for separating DNA and RNA molecules. It is often preferred over other buffers, such as TAE buffer, for its ability to resolve small DNA fragments more effectively.
What is the concentration of TBE buffer (10X) and how is it prepared for use?
TBE buffer is typically supplied as a 10X concentrated solution, which means it needs to be diluted before use. To prepare a 1X working solution, simply dilute the 10X stock solution with distilled water at a 1:10 ratio. For example, mix 100 ml of 10X TBE buffer with 900 ml of distilled water to make 1 liter of 1X TBE buffer.
Can TBE buffer (10X) be used for other applications besides nucleic acid electrophoresis?
TBE buffer is primarily used for DNA and RNA electrophoresis, but it can also be used for other applications such as running protein gels for SDS-PAGE. However, TBE buffer is not recommended for protein electrophoresis as it may interfere with protein migration and resolution.
Are there any precautions or special storage requirements for TBE buffer (10X)?
TBE buffer should be stored at room temperature and protected from light to prevent degradation. It is also important to avoid contamination of the buffer with nucleases or other impurities that may affect the quality of electrophoresis results. Additionally, always wear appropriate personal protective equipment when handling TBE buffer to avoid skin or eye irritation.
