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| Name | TBE buffer (10X) powder |
| Volume | 1 kg |
| Concentration | 10X |
| Application | electrophoresis |
| Storage Condition | room temperature |
| PH Range | 7.6-8.0 |
| Shelf Life | 2 years |
| Composition | Tris, boric acid, EDTA |
| Solubility | soluble in water |
| Usage | dissolve in water to make 1X TBE buffer |
| Packaging | resealable pouch |
FAQ
What is TBE buffer (10X) powder and its purpose?
TBE buffer (10X) powder is a widely used buffer solution in molecular biology and biochemistry applications. It is commonly used for agarose and polyacrylamide gel electrophoresis, as well as for DNA and RNA electrophoresis. The buffer helps to maintain the pH of the solution, stabilize nucleic acids, and provide ions for conducting electricity during electrophoresis.
How is TBE buffer (10X) powder prepared for use?
To prepare TBE buffer (1X) solution, mix 100 mL of TBE buffer (10X) powder with 900 mL of distilled water. Stir the solution until the powder is completely dissolved. The resulting solution is now 1X TBE buffer and is ready for use in electrophoresis experiments.
What are the advantages of using TBE buffer (10X) powder over other buffer solutions?
TBE buffer (10X) powder has several advantages over other buffer solutions. It has a high buffering capacity, which helps maintain a stable pH during electrophoresis. It also provides good resolution of DNA and RNA bands, making it ideal for separating and analyzing nucleic acids. Additionally, TBE buffer is easy to prepare and store, making it a convenient choice for researchers.
Can TBE buffer (10X) powder be used for other applications besides electrophoresis?
While TBE buffer (10X) powder is primarily used for electrophoresis, it can also be used for other applications in molecular biology and biochemistry. For example, TBE buffer can be used as a running buffer for protein electrophoresis, as a buffer for nucleic acid purification, or as a buffer for DNA and RNA hybridization experiments. Its versatility makes it a useful tool for a variety of laboratory techniques.
What precautions should be taken when using TBE buffer (10X) powder?
When using TBE buffer (10X) powder, it is important to follow standard laboratory safety protocols. Avoid direct contact with the powder, as it can irritate the skin and eyes. Always wear appropriate personal protective equipment, such as gloves and goggles, when handling the powder. Additionally, store the powder in a cool, dry place away from direct sunlight. If the powder comes into contact with skin or eyes, rinse immediately with water and seek medical attention if irritation persists.
TBE buffer (10X) powder is a widely used buffer solution in molecular biology and biochemistry applications. It is commonly used for agarose and polyacrylamide gel electrophoresis, as well as for DNA and RNA electrophoresis. The buffer helps to maintain the pH of the solution, stabilize nucleic acids, and provide ions for conducting electricity during electrophoresis.
How is TBE buffer (10X) powder prepared for use?
To prepare TBE buffer (1X) solution, mix 100 mL of TBE buffer (10X) powder with 900 mL of distilled water. Stir the solution until the powder is completely dissolved. The resulting solution is now 1X TBE buffer and is ready for use in electrophoresis experiments.
What are the advantages of using TBE buffer (10X) powder over other buffer solutions?
TBE buffer (10X) powder has several advantages over other buffer solutions. It has a high buffering capacity, which helps maintain a stable pH during electrophoresis. It also provides good resolution of DNA and RNA bands, making it ideal for separating and analyzing nucleic acids. Additionally, TBE buffer is easy to prepare and store, making it a convenient choice for researchers.
Can TBE buffer (10X) powder be used for other applications besides electrophoresis?
While TBE buffer (10X) powder is primarily used for electrophoresis, it can also be used for other applications in molecular biology and biochemistry. For example, TBE buffer can be used as a running buffer for protein electrophoresis, as a buffer for nucleic acid purification, or as a buffer for DNA and RNA hybridization experiments. Its versatility makes it a useful tool for a variety of laboratory techniques.
What precautions should be taken when using TBE buffer (10X) powder?
When using TBE buffer (10X) powder, it is important to follow standard laboratory safety protocols. Avoid direct contact with the powder, as it can irritate the skin and eyes. Always wear appropriate personal protective equipment, such as gloves and goggles, when handling the powder. Additionally, store the powder in a cool, dry place away from direct sunlight. If the powder comes into contact with skin or eyes, rinse immediately with water and seek medical attention if irritation persists.
