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| Name | TBE buffer (5X) |
| Use | Electrophoresis of nucleic acids |
| Concentration | 5X |
| PH | 8.3 |
| Components | Tris base, Boric acid, EDTA |
| Storage | Room temperature |
| Stability | Stable for long term storage |
| Volume | 1 L |
| Appearance | Clear colorless liquid |
| Safety | Avoid contact with skin and eyes |
| Applications | Agarose gel electrophoresis |
| DNase | RNase-free |
FAQ
What is TBE buffer?
TBE buffer, also known as Tris-Borate-EDTA buffer, is a commonly used buffer solution in molecular biology for various applications such as agarose gel electrophoresis, DNA and RNA gel extraction, and nucleic acid sample preparation.
What are the components of TBE buffer?
TBE buffer typically contains Tris base, boric acid, and EDTA (ethylenediaminetetraacetic acid). These components work together to maintain a stable pH and provide the necessary ions for conducting electrophoresis experiments effectively.
What is the concentration of TBE buffer in the 5X solution?
The 5X TBE buffer solution is five times more concentrated than a 1X solution, with a typical composition of 0.4 M Tris base, 0.4 M boric acid, and 10 mM EDTA. This higher concentration is often preferred for faster running times and better resolution in gel electrophoresis.
How is TBE buffer used in agarose gel electrophoresis?
In agarose gel electrophoresis, TBE buffer is used as both the running buffer in the gel tank and as the gel matrix for separating nucleic acids (DNA or RNA). The buffer provides the necessary ions for conducting electricity through the gel, allowing the negatively charged nucleic acids to migrate towards the positive electrode based on size.
Can TBE buffer be used for RNA applications?
Yes, TBE buffer is suitable for RNA applications, including RNA gel electrophoresis and RNA extraction. The EDTA in TBE buffer helps to chelate divalent metal ions, which can degrade RNA samples, making it a preferred choice for RNA-based experiments.
TBE buffer, also known as Tris-Borate-EDTA buffer, is a commonly used buffer solution in molecular biology for various applications such as agarose gel electrophoresis, DNA and RNA gel extraction, and nucleic acid sample preparation.
What are the components of TBE buffer?
TBE buffer typically contains Tris base, boric acid, and EDTA (ethylenediaminetetraacetic acid). These components work together to maintain a stable pH and provide the necessary ions for conducting electrophoresis experiments effectively.
What is the concentration of TBE buffer in the 5X solution?
The 5X TBE buffer solution is five times more concentrated than a 1X solution, with a typical composition of 0.4 M Tris base, 0.4 M boric acid, and 10 mM EDTA. This higher concentration is often preferred for faster running times and better resolution in gel electrophoresis.
How is TBE buffer used in agarose gel electrophoresis?
In agarose gel electrophoresis, TBE buffer is used as both the running buffer in the gel tank and as the gel matrix for separating nucleic acids (DNA or RNA). The buffer provides the necessary ions for conducting electricity through the gel, allowing the negatively charged nucleic acids to migrate towards the positive electrode based on size.
Can TBE buffer be used for RNA applications?
Yes, TBE buffer is suitable for RNA applications, including RNA gel electrophoresis and RNA extraction. The EDTA in TBE buffer helps to chelate divalent metal ions, which can degrade RNA samples, making it a preferred choice for RNA-based experiments.
