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| Name | TE buffer (1X) pH 8.0 |
| Type | Molecular biology product |
| PH | 8 |
| Concentration | 1X |
| Use | DNA and RNA sample storage and dilution |
| Composition | Tris and EDTA |
| Storage Conditions | Store at room temperature |
| Stability | Stable for at least 1 year |
| Sterilization | Filter sterilized |
| Color | Clear |
| Solubility | Soluble in water |
| Manufacturing Method | Chemically synthesized |
| Quantity | 500 mL |
| Shelf Life | 2 years |
| Applications | Molecular biology experiments |
| Supplier | Various |
| Shipping Conditions | Shipped at room temperature |
FAQ
What is TE buffer (1X) pH 8.0 and why is it used in molecular biology?
TE buffer (1X) pH 8.0 is a standard buffer solution used in molecular biology for various applications such as DNA and RNA extraction, molecular cloning, and PCR reactions. It consists of Tris-HCl (pH 8.0) and EDTA, which help maintain the pH of the solution and prevent nucleases from degrading nucleic acids.
How is TE buffer (1X) pH 8.0 prepared and stored for molecular biology experiments?
TE buffer (1X) pH 8.0 can be easily prepared by mixing the appropriate amounts of Tris-HCl and EDTA in water to achieve the desired concentration. It is important to adjust the pH to 8.0 using HCl or NaOH if necessary. The solution can be stored at room temperature for short-term use or at 4°C for long-term storage.
What are the advantages of using TE buffer (1X) pH 8.0 in molecular biology experiments?
TE buffer (1X) pH 8.0 helps protect DNA and RNA from degradation by nucleases present in the environment. It also provides a stable pH environment for enzymatic reactions such as restriction digestion and PCR. Additionally, TE buffer (1X) pH 8.0 can be used to dilute nucleic acid samples before analysis by gel electrophoresis.
Can TE buffer (1X) pH 8.0 be used for RNA extraction and purification in molecular biology?
Yes, TE buffer (1X) pH 8.0 can be used for RNA extraction and purification by solubilizing RNA and protecting it from degradation. However, it is important to note that EDTA can inhibit some enzymes used in RNA isolation, so it is recommended to perform a final wash step using a buffer without EDTA for RNA purification.
What precautions should be taken when using TE buffer (1X) pH 8.0 in molecular biology experiments?
It is important to use nuclease-free water and reagents when preparing TE buffer (1X) pH 8.0 to avoid contamination and degradation of nucleic acids. Additionally, proper storage of the buffer at the recommended temperature will help maintain its stability and effectiveness in molecular biology applications.
TE buffer (1X) pH 8.0 is a standard buffer solution used in molecular biology for various applications such as DNA and RNA extraction, molecular cloning, and PCR reactions. It consists of Tris-HCl (pH 8.0) and EDTA, which help maintain the pH of the solution and prevent nucleases from degrading nucleic acids.
How is TE buffer (1X) pH 8.0 prepared and stored for molecular biology experiments?
TE buffer (1X) pH 8.0 can be easily prepared by mixing the appropriate amounts of Tris-HCl and EDTA in water to achieve the desired concentration. It is important to adjust the pH to 8.0 using HCl or NaOH if necessary. The solution can be stored at room temperature for short-term use or at 4°C for long-term storage.
What are the advantages of using TE buffer (1X) pH 8.0 in molecular biology experiments?
TE buffer (1X) pH 8.0 helps protect DNA and RNA from degradation by nucleases present in the environment. It also provides a stable pH environment for enzymatic reactions such as restriction digestion and PCR. Additionally, TE buffer (1X) pH 8.0 can be used to dilute nucleic acid samples before analysis by gel electrophoresis.
Can TE buffer (1X) pH 8.0 be used for RNA extraction and purification in molecular biology?
Yes, TE buffer (1X) pH 8.0 can be used for RNA extraction and purification by solubilizing RNA and protecting it from degradation. However, it is important to note that EDTA can inhibit some enzymes used in RNA isolation, so it is recommended to perform a final wash step using a buffer without EDTA for RNA purification.
What precautions should be taken when using TE buffer (1X) pH 8.0 in molecular biology experiments?
It is important to use nuclease-free water and reagents when preparing TE buffer (1X) pH 8.0 to avoid contamination and degradation of nucleic acids. Additionally, proper storage of the buffer at the recommended temperature will help maintain its stability and effectiveness in molecular biology applications.
