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| Name | TE buffer (1X) pH 8.0 low EDTA |
| Category | Molecular biology product |
| PH | 8 |
| Concentration | 1X |
| Contains | low EDTA |
| Application | DNA and RNA storage and resuspension |
| Storage Condition | Store at room temperature |
| Composition | Tris and EDTA in deionized water |
| Volume | Varies |
| Color | Colorless |
| Packaging | Bottles or tubes |
| Sterilization | Sterile filtered |
| Compatibility | Compatible with most molecular biology applications |
| Shelf Life | 1 year |
| Manufacturer | Various manufacturers |
| Shipping Condition | Shipped at room temperature |
| Stability | Stable under recommended storage conditions |
| Usage | For laboratory use only |
| Regulatory Status | Not for diagnostic use |
| Specificity | Specific for DNA and RNA applications |
| QC Testing | Quality control tested |
| Lot Specific Information | Lot-specific information available on request |
FAQ
What is TE buffer (1X) pH 8.0 with low EDTA used for in molecular biology?
TE buffer (Tris-EDTA buffer) is a commonly used buffer solution in molecular biology for various applications such as DNA and RNA purification, storage, and preservation. It is specifically designed to protect nucleic acids from degradation. The 1X concentration refers to a standardized dilution used for most molecular biology applications, while pH 8.0 is the optimal pH for stability and functionality of nucleic acids. The low EDTA concentration helps to chelate divalent cations that could otherwise degrade nucleic acids.
How is TE buffer (1X) pH 8.0 with low EDTA prepared and stored?
TE buffer (1X) pH 8.0 with low EDTA can be prepared by mixing Tris base, EDTA, and adjusting the pH to 8.0 with hydrochloric acid. The buffer solution should be sterilized by filtration or autoclaving before use to prevent contamination. It can be stored at room temperature, preferably in a dark container to avoid light exposure that can degrade the buffer components. It is important to aliquot the buffer into smaller volumes to prevent contamination and repeated freeze-thaw cycles.
What are the advantages of using TE buffer (1X) pH 8.0 with low EDTA over other buffer solutions in molecular biology?
TE buffer (1X) pH 8.0 with low EDTA offers several advantages over other buffer solutions in molecular biology. Firstly, it provides an ideal pH environment for nucleic acids, ensuring their stability and functionality. The low EDTA concentration helps to chelate divalent cations that could otherwise degrade nucleic acids. Additionally, TE buffer is versatile and can be used for DNA and RNA purification, storage, and preservation. Its standardized 1X concentration makes it easy to use for most molecular biology applications.
In what applications can TE buffer (1X) pH 8.0 with low EDTA be used?
TE buffer (1X) pH 8.0 with low EDTA can be used in various molecular biology applications, including DNA and RNA purification, storage, and preservation. It is commonly used in nucleic acid extraction protocols, PCR reactions, DNA sequencing, restriction enzyme digestion, and other molecular biology techniques where maintaining the integrity of nucleic acids is crucial. The buffer solution is also suitable for long-term storage of DNA and RNA samples due to its ability to protect nucleic acids from degradation.
How does TE buffer (1X) pH 8.0 with low EDTA help in protecting nucleic acids during molecular biology experiments?
TE buffer (1X) pH 8.0 with low EDTA helps in protecting nucleic acids during molecular biology experiments by providing a stable pH environment that minimizes degradation. The low EDTA concentration in the buffer chelates divalent cations, such as Mg2+, that can catalyze the breakdown of nucleic acids. This helps to preserve the integrity of DNA and RNA samples, ensuring accurate and reliable results in various molecular biology techniques. Additionally, TE buffer is used for long-term storage of nucleic acids, preventing degradation over time.
TE buffer (Tris-EDTA buffer) is a commonly used buffer solution in molecular biology for various applications such as DNA and RNA purification, storage, and preservation. It is specifically designed to protect nucleic acids from degradation. The 1X concentration refers to a standardized dilution used for most molecular biology applications, while pH 8.0 is the optimal pH for stability and functionality of nucleic acids. The low EDTA concentration helps to chelate divalent cations that could otherwise degrade nucleic acids.
How is TE buffer (1X) pH 8.0 with low EDTA prepared and stored?
TE buffer (1X) pH 8.0 with low EDTA can be prepared by mixing Tris base, EDTA, and adjusting the pH to 8.0 with hydrochloric acid. The buffer solution should be sterilized by filtration or autoclaving before use to prevent contamination. It can be stored at room temperature, preferably in a dark container to avoid light exposure that can degrade the buffer components. It is important to aliquot the buffer into smaller volumes to prevent contamination and repeated freeze-thaw cycles.
What are the advantages of using TE buffer (1X) pH 8.0 with low EDTA over other buffer solutions in molecular biology?
TE buffer (1X) pH 8.0 with low EDTA offers several advantages over other buffer solutions in molecular biology. Firstly, it provides an ideal pH environment for nucleic acids, ensuring their stability and functionality. The low EDTA concentration helps to chelate divalent cations that could otherwise degrade nucleic acids. Additionally, TE buffer is versatile and can be used for DNA and RNA purification, storage, and preservation. Its standardized 1X concentration makes it easy to use for most molecular biology applications.
In what applications can TE buffer (1X) pH 8.0 with low EDTA be used?
TE buffer (1X) pH 8.0 with low EDTA can be used in various molecular biology applications, including DNA and RNA purification, storage, and preservation. It is commonly used in nucleic acid extraction protocols, PCR reactions, DNA sequencing, restriction enzyme digestion, and other molecular biology techniques where maintaining the integrity of nucleic acids is crucial. The buffer solution is also suitable for long-term storage of DNA and RNA samples due to its ability to protect nucleic acids from degradation.
How does TE buffer (1X) pH 8.0 with low EDTA help in protecting nucleic acids during molecular biology experiments?
TE buffer (1X) pH 8.0 with low EDTA helps in protecting nucleic acids during molecular biology experiments by providing a stable pH environment that minimizes degradation. The low EDTA concentration in the buffer chelates divalent cations, such as Mg2+, that can catalyze the breakdown of nucleic acids. This helps to preserve the integrity of DNA and RNA samples, ensuring accurate and reliable results in various molecular biology techniques. Additionally, TE buffer is used for long-term storage of nucleic acids, preventing degradation over time.
