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| Name | Tris Hydrochloride for buffer solutions |
| Appearance | White crystalline powder |
| Purity | 99% |
| Molecular Formula | C4H11NO3·HCl |
| Molecular Weight | 157.60 g/mol |
| Solubility | Soluble in water |
| Storage Condition | Store at room temperature |
| PH Range | 7-9 |
| Usage | Commonly used as a buffer in biochemical and molecular biology research |
| Cas Number | 1185-53-1 |
| Shelf Life | 2 years |
| Applications | Used in protein crystallization, enzyme assays, and electrophoresis |
FAQ
What is Tris Hydrochloride and how is it used in buffer solutions?
Tris Hydrochloride, also known as Tris HCl, is a common buffer used in laboratory settings to maintain a stable pH level in various chemical and biological experiments. It is a salt form of Tris base and hydrochloric acid, providing buffering capacity in the range of pH 7-9.
Why is Tris Hydrochloride preferred for buffer solutions?
Tris Hydrochloride is preferred for buffer solutions due to its minimal interference with biochemical reactions, high solubility in water, and pH stability over a wide range. It is also cost-effective and readily available in the market, making it a popular choice among researchers and scientists.
How is Tris Hydrochloride prepared and diluted for use in buffer solutions?
Tris Hydrochloride can be prepared by dissolving the desired amount of the salt in distilled water and adjusting the pH with either hydrochloric acid or sodium hydroxide. The concentration of Tris Hydrochloride in the buffer solution can be adjusted by diluting the stock solution with water to achieve the desired pH and buffering capacity.
What are the applications of Tris Hydrochloride in laboratory experiments?
Tris Hydrochloride is commonly used in molecular biology, biochemistry, and cell culture experiments as a buffering agent to maintain a constant pH level. It is also used in protein electrophoresis, enzyme assays, and DNA gel electrophoresis, where pH stability is crucial for the success of the experiment.
Are there any precautions or storage requirements for Tris Hydrochloride?
It is recommended to store Tris Hydrochloride in a dry and cool environment away from direct sunlight to prevent degradation. When handling the salt, it is important to wear appropriate personal protective equipment such as gloves and goggles to avoid skin contact or inhalation of fumes. Additionally, Tris Hydrochloride should be kept away from acids, oxidizing agents, and metal ions to maintain its integrity and effectiveness as a buffer solution.
Tris Hydrochloride, also known as Tris HCl, is a common buffer used in laboratory settings to maintain a stable pH level in various chemical and biological experiments. It is a salt form of Tris base and hydrochloric acid, providing buffering capacity in the range of pH 7-9.
Why is Tris Hydrochloride preferred for buffer solutions?
Tris Hydrochloride is preferred for buffer solutions due to its minimal interference with biochemical reactions, high solubility in water, and pH stability over a wide range. It is also cost-effective and readily available in the market, making it a popular choice among researchers and scientists.
How is Tris Hydrochloride prepared and diluted for use in buffer solutions?
Tris Hydrochloride can be prepared by dissolving the desired amount of the salt in distilled water and adjusting the pH with either hydrochloric acid or sodium hydroxide. The concentration of Tris Hydrochloride in the buffer solution can be adjusted by diluting the stock solution with water to achieve the desired pH and buffering capacity.
What are the applications of Tris Hydrochloride in laboratory experiments?
Tris Hydrochloride is commonly used in molecular biology, biochemistry, and cell culture experiments as a buffering agent to maintain a constant pH level. It is also used in protein electrophoresis, enzyme assays, and DNA gel electrophoresis, where pH stability is crucial for the success of the experiment.
Are there any precautions or storage requirements for Tris Hydrochloride?
It is recommended to store Tris Hydrochloride in a dry and cool environment away from direct sunlight to prevent degradation. When handling the salt, it is important to wear appropriate personal protective equipment such as gloves and goggles to avoid skin contact or inhalation of fumes. Additionally, Tris Hydrochloride should be kept away from acids, oxidizing agents, and metal ions to maintain its integrity and effectiveness as a buffer solution.
