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| Name | Tris Hydrochloride |
| Product Type | Molecular Biology Reagent |
| Form | Powder |
| Molecular Weight | 157.60 g/mol |
| Purity | ≥99% |
| Solubility | Soluble in water |
| Storage Conditions | Store at room temperature |
| Shelf Life | 2 years |
| Applications | Buffering agent, protein electrophoresis |
| Cas Number | 1185-53-1 |
| Appearance | White crystalline powder |
| Melting Point | 167-172°C |
| Boiling Point | 219-220°C |
| Density | 1.67 g/cm3 |
| Flash Point | 187°C |
| Usage Recommendation | Dissolve in water to desired concentration for use |
FAQ
What is Tris Hydrochloride and what is its role in molecular biology?
Tris Hydrochloride is a buffer commonly used in molecular biology experiments. It helps maintain a stable pH level, which is crucial for many enzymatic reactions and other biological processes that occur in the laboratory.
How is Tris Hydrochloride prepared and used in experiments?
Tris Hydrochloride is typically prepared as a stock solution by dissolving the powder in water to achieve the desired concentration. It can then be added to various solutions to act as a buffer and maintain the pH at a specific level throughout an experiment.
What are the advantages of using Tris Hydrochloride in molecular biology research?
Tris Hydrochloride offers several benefits in molecular biology research, including its ability to maintain a stable pH, its compatibility with a wide range of biological reactions, and its cost-effectiveness compared to other buffers on the market.
Are there any specific considerations to keep in mind when using Tris Hydrochloride in experiments?
While Tris Hydrochloride is a versatile buffer, researchers should be aware of its limited solubility at high concentrations and its potential to interfere with certain enzymatic reactions. It is important to carefully follow the recommended protocols and guidelines for using this buffer in experiments.
In what types of molecular biology experiments is Tris Hydrochloride most commonly used?
Tris Hydrochloride is commonly used in a variety of molecular biology experiments, including PCR, gel electrophoresis, Western blotting, and protein purification. Its versatility and effectiveness make it a staple in many research laboratories.
Tris Hydrochloride is a buffer commonly used in molecular biology experiments. It helps maintain a stable pH level, which is crucial for many enzymatic reactions and other biological processes that occur in the laboratory.
How is Tris Hydrochloride prepared and used in experiments?
Tris Hydrochloride is typically prepared as a stock solution by dissolving the powder in water to achieve the desired concentration. It can then be added to various solutions to act as a buffer and maintain the pH at a specific level throughout an experiment.
What are the advantages of using Tris Hydrochloride in molecular biology research?
Tris Hydrochloride offers several benefits in molecular biology research, including its ability to maintain a stable pH, its compatibility with a wide range of biological reactions, and its cost-effectiveness compared to other buffers on the market.
Are there any specific considerations to keep in mind when using Tris Hydrochloride in experiments?
While Tris Hydrochloride is a versatile buffer, researchers should be aware of its limited solubility at high concentrations and its potential to interfere with certain enzymatic reactions. It is important to carefully follow the recommended protocols and guidelines for using this buffer in experiments.
In what types of molecular biology experiments is Tris Hydrochloride most commonly used?
Tris Hydrochloride is commonly used in a variety of molecular biology experiments, including PCR, gel electrophoresis, Western blotting, and protein purification. Its versatility and effectiveness make it a staple in many research laboratories.
