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| Name | Tris buffer pH 8.0 (1 M) |
| Product Type | Molecular biology reagent |
| Ph | 8 |
| Concentration | 1 M |
| Buffer Type | Tris |
| Purity | High purity |
| Storage Conditions | Store at room temperature |
| Solubility | Soluble in water |
| Usage | Commonly used in molecular biology experiments |
| Stability | Stable at room temperature |
| Application | Used for DNA and RNA extraction, protein purification, and enzyme assays |
| Compatibility | Compatible with a wide range of enzymes and proteins |
| Manufacturing Source | Synthetic source |
| Shelf Life | 2 years |
| Package Size | 500 mL |
| Chemical Formula | C4H11NO3 |
| Molecular Weight | 121.14 g/mol |
FAQ
What is Tris buffer pH 8.0 (1 M) and how is it used in molecular biology?
Tris buffer pH 8.0 (1 M) is a commonly used buffer solution in molecular biology. It is composed of tris(hydroxymethyl)aminomethane, which is a compound that helps maintain a stable pH level in biological reactions. The pH of 8.0 is ideal for many molecular biology applications, as it falls within the physiological range for most biological systems.
What are some of the key features and benefits of using Tris buffer pH 8.0 (1 M) in molecular biology experiments?
One of the main benefits of using Tris buffer pH 8.0 (1 M) is its ability to maintain a stable pH level, which is critical for ensuring the success of molecular biology experiments. Additionally, Tris buffer is known for its high solubility and compatibility with a wide range of molecules and biological samples. Its 1 M concentration also makes it easy to dilute to lower concentrations for specific experimental requirements.
How is Tris buffer pH 8.0 (1 M) typically prepared and stored for use in molecular biology experiments?
To prepare Tris buffer pH 8.0 (1 M), simply dissolve the appropriate amount of Tris base in water and adjust the pH using hydrochloric acid. The buffer solution can be stored at room temperature for short-term use, or aliquoted and stored at -20°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to maintain the integrity of the buffer solution.
In what types of molecular biology applications is Tris buffer pH 8.0 (1 M) commonly used?
Tris buffer pH 8.0 (1 M) is versatile and can be used in a wide range of molecular biology applications, including DNA and RNA isolation, PCR, electrophoresis, and protein purification. Its stability and compatibility with various enzymes and biological molecules make it an ideal choice for many experimental setups.
Are there any specific precautions or considerations to keep in mind when using Tris buffer pH 8.0 (1 M) in molecular biology experiments?
While Tris buffer pH 8.0 (1 M) is generally safe to use, it is important to handle it with care and follow standard laboratory safety protocols. Avoid contact with skin or eyes, and wear appropriate personal protective equipment when working with the buffer solution. Additionally, always ensure that the pH of the buffer is accurately adjusted before using it in experiments to avoid any unwanted effects on the biological reactions.
Tris buffer pH 8.0 (1 M) is a commonly used buffer solution in molecular biology. It is composed of tris(hydroxymethyl)aminomethane, which is a compound that helps maintain a stable pH level in biological reactions. The pH of 8.0 is ideal for many molecular biology applications, as it falls within the physiological range for most biological systems.
What are some of the key features and benefits of using Tris buffer pH 8.0 (1 M) in molecular biology experiments?
One of the main benefits of using Tris buffer pH 8.0 (1 M) is its ability to maintain a stable pH level, which is critical for ensuring the success of molecular biology experiments. Additionally, Tris buffer is known for its high solubility and compatibility with a wide range of molecules and biological samples. Its 1 M concentration also makes it easy to dilute to lower concentrations for specific experimental requirements.
How is Tris buffer pH 8.0 (1 M) typically prepared and stored for use in molecular biology experiments?
To prepare Tris buffer pH 8.0 (1 M), simply dissolve the appropriate amount of Tris base in water and adjust the pH using hydrochloric acid. The buffer solution can be stored at room temperature for short-term use, or aliquoted and stored at -20°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to maintain the integrity of the buffer solution.
In what types of molecular biology applications is Tris buffer pH 8.0 (1 M) commonly used?
Tris buffer pH 8.0 (1 M) is versatile and can be used in a wide range of molecular biology applications, including DNA and RNA isolation, PCR, electrophoresis, and protein purification. Its stability and compatibility with various enzymes and biological molecules make it an ideal choice for many experimental setups.
Are there any specific precautions or considerations to keep in mind when using Tris buffer pH 8.0 (1 M) in molecular biology experiments?
While Tris buffer pH 8.0 (1 M) is generally safe to use, it is important to handle it with care and follow standard laboratory safety protocols. Avoid contact with skin or eyes, and wear appropriate personal protective equipment when working with the buffer solution. Additionally, always ensure that the pH of the buffer is accurately adjusted before using it in experiments to avoid any unwanted effects on the biological reactions.
