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| Product Name | Tris for buffer solutions |
| Usage | Used as a buffering agent in biochemical and molecular biology research |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| PH Range | 7.5 - 9.0 |
| Storage | Store in a cool, dry place |
| Stability | Stable under normal conditions |
| Melting Point | 169-172°C |
| Formula | C4H11NO3 |
| Molar Mass | 121.14 g/mol |
| CAS Number | 77-86-1 |
FAQ
What are Tris buffer solutions and what are they used for?
Tris buffer solutions are mixtures of the chemical compound Tris(hydroxymethyl)aminomethane (Tris) and water. They are commonly used in biochemical and molecular biology laboratories to control the pH of solutions. Tris buffer solutions have a high buffering capacity, meaning they can maintain a stable pH even when small amounts of acid or base are added.
What are the advantages of using Tris buffer solutions?
One of the main advantages of using Tris buffer solutions is their ability to maintain a stable pH over a wide range of temperatures. This makes them ideal for experiments that require precise pH control. Tris buffer solutions are also non-toxic and have a low ionic strength, making them suitable for a variety of biological applications.
How do I prepare a Tris buffer solution?
To prepare a Tris buffer solution, you will need to dissolve the appropriate amount of Tris base in water and adjust the pH to the desired value using a strong acid such as hydrochloric acid or a strong base such as sodium hydroxide. The exact procedure will depend on the specific concentration and pH needed for your experiment, so it is important to follow a reliable protocol or consult a reference book.
Can Tris buffer solutions be autoclaved?
Yes, Tris buffer solutions can be autoclaved without any adverse effects on their buffering capacity or pH stability. However, it is important to ensure that the containers used for autoclaving are suitable for high heat and pressure, and that the solutions are allowed to cool down properly before use. Autoclaving is a common method for sterilizing solutions in laboratory settings.
What is the shelf life of Tris buffer solutions?
The shelf life of Tris buffer solutions can vary depending on the specific formulation and storage conditions. In general, Tris buffer solutions stored at room temperature in a tightly sealed container can remain stable for several months to a year. It is important to monitor the pH of the solution periodically and discard any solutions that show signs of contamination or pH drift. For longer-term storage, Tris buffer solutions can be kept in the refrigerator or freezer to extend their shelf life.
Tris buffer solutions are mixtures of the chemical compound Tris(hydroxymethyl)aminomethane (Tris) and water. They are commonly used in biochemical and molecular biology laboratories to control the pH of solutions. Tris buffer solutions have a high buffering capacity, meaning they can maintain a stable pH even when small amounts of acid or base are added.
What are the advantages of using Tris buffer solutions?
One of the main advantages of using Tris buffer solutions is their ability to maintain a stable pH over a wide range of temperatures. This makes them ideal for experiments that require precise pH control. Tris buffer solutions are also non-toxic and have a low ionic strength, making them suitable for a variety of biological applications.
How do I prepare a Tris buffer solution?
To prepare a Tris buffer solution, you will need to dissolve the appropriate amount of Tris base in water and adjust the pH to the desired value using a strong acid such as hydrochloric acid or a strong base such as sodium hydroxide. The exact procedure will depend on the specific concentration and pH needed for your experiment, so it is important to follow a reliable protocol or consult a reference book.
Can Tris buffer solutions be autoclaved?
Yes, Tris buffer solutions can be autoclaved without any adverse effects on their buffering capacity or pH stability. However, it is important to ensure that the containers used for autoclaving are suitable for high heat and pressure, and that the solutions are allowed to cool down properly before use. Autoclaving is a common method for sterilizing solutions in laboratory settings.
What is the shelf life of Tris buffer solutions?
The shelf life of Tris buffer solutions can vary depending on the specific formulation and storage conditions. In general, Tris buffer solutions stored at room temperature in a tightly sealed container can remain stable for several months to a year. It is important to monitor the pH of the solution periodically and discard any solutions that show signs of contamination or pH drift. For longer-term storage, Tris buffer solutions can be kept in the refrigerator or freezer to extend their shelf life.
