Back
| Product Name | ß-Mercaptoethanol |
| Product Type | Reagent |
| Application | Molecular Biology |
| CAS Number | 60-24-2 |
| Molecular Formula | C2H6OS |
| Molar Mass | 78.134 g/mol |
| Appearance | Colorless liquid |
| Odor | Unpleasant, like rotten cabbage |
| Storage Conditions | Store at room temperature away from light |
| Solubility | Miscible in water, ethanol, and ether |
| Density | 1.114 g/cm3 |
| Boiling Point | 99 °C |
| Melting Point | -48 °C |
| Purity | ≥ 99% |
| Stability | Stable under recommended storage conditions |
| Hazard Statements | Causes skin irritation, causes serious eye irritation, harmful if swallowed |
| Safety Precautions | Wear protective gloves/eye protection, wash thoroughly after handling, do not eat, drink or smoke while using |
FAQ
What is ß-Mercaptoethanol and how is it used in molecular biology?
ß-Mercaptoethanol, often abbreviated as BME, is a reducing agent commonly used in molecular biology experiments. It helps to break disulfide bonds, which are important for protein structure, allowing for the denaturation of proteins. In molecular biology, ß-Mercaptoethanol is frequently used in processes such as protein denaturation, Western blotting, and RNA sample preparation.
What are the benefits of using ß-Mercaptoethanol in molecular biology experiments?
One of the main benefits of using ß-Mercaptoethanol in molecular biology experiments is its ability to reduce disulfide bonds in proteins. This helps to denature proteins, making it easier to study their structure and function. Additionally, ß-Mercaptoethanol can help to prevent the oxidation of proteins, which can affect the results of experiments.
How is ß-Mercaptoethanol typically used in molecular biology experiments?
ß-Mercaptoethanol is typically used in molecular biology experiments as a reducing agent to denature proteins. It is often added to protein samples before running them on a gel for techniques like SDS-PAGE or Western blotting. Additionally, it can be used in RNA sample preparation to prevent RNA degradation.
Are there any precautions or considerations to keep in mind when using ß-Mercaptoethanol in molecular biology experiments?
Yes, there are some precautions to keep in mind when using ß-Mercaptoethanol in molecular biology experiments. It is important to use it in a well-ventilated area, as it has a strong odor and can be harmful if inhaled. Additionally, it should be handled with care, as it can cause skin and eye irritation. It is also important to store it properly and avoid mixing it with incompatible chemicals.
Where can I purchase high-quality ß-Mercaptoethanol for my molecular biology experiments?
High-quality ß-Mercaptoethanol can be purchased from reputable suppliers of laboratory chemicals and reagents. It is important to choose a supplier that offers ß-Mercaptoethanol that is of a high purity and suitable for use in molecular biology experiments. Additionally, it is recommended to check the expiry date and storage conditions of the ß-Mercaptoethanol before purchasing it.
ß-Mercaptoethanol, often abbreviated as BME, is a reducing agent commonly used in molecular biology experiments. It helps to break disulfide bonds, which are important for protein structure, allowing for the denaturation of proteins. In molecular biology, ß-Mercaptoethanol is frequently used in processes such as protein denaturation, Western blotting, and RNA sample preparation.
What are the benefits of using ß-Mercaptoethanol in molecular biology experiments?
One of the main benefits of using ß-Mercaptoethanol in molecular biology experiments is its ability to reduce disulfide bonds in proteins. This helps to denature proteins, making it easier to study their structure and function. Additionally, ß-Mercaptoethanol can help to prevent the oxidation of proteins, which can affect the results of experiments.
How is ß-Mercaptoethanol typically used in molecular biology experiments?
ß-Mercaptoethanol is typically used in molecular biology experiments as a reducing agent to denature proteins. It is often added to protein samples before running them on a gel for techniques like SDS-PAGE or Western blotting. Additionally, it can be used in RNA sample preparation to prevent RNA degradation.
Are there any precautions or considerations to keep in mind when using ß-Mercaptoethanol in molecular biology experiments?
Yes, there are some precautions to keep in mind when using ß-Mercaptoethanol in molecular biology experiments. It is important to use it in a well-ventilated area, as it has a strong odor and can be harmful if inhaled. Additionally, it should be handled with care, as it can cause skin and eye irritation. It is also important to store it properly and avoid mixing it with incompatible chemicals.
Where can I purchase high-quality ß-Mercaptoethanol for my molecular biology experiments?
High-quality ß-Mercaptoethanol can be purchased from reputable suppliers of laboratory chemicals and reagents. It is important to choose a supplier that offers ß-Mercaptoethanol that is of a high purity and suitable for use in molecular biology experiments. Additionally, it is recommended to check the expiry date and storage conditions of the ß-Mercaptoethanol before purchasing it.
