Acrylamide - Solution (30 %) - Mix 29 : 1 for molecular biology

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Product Name Acrylamide - Solution (30%) - Mix 29 : 1
Product Type Molecular biology reagent
Concentration 30%
Mix Ratio 29:1
Appearance Clear colorless liquid
Solubility Soluble in water
Usage Used for polyacrylamide gel electrophoresis
Storage Conditions Store at room temperature
Stability Stable for long-term storage
Hazard Warnings Causes skin irritation, may cause respiratory irritation
Safety Precautions Wear protective gloves and clothing, avoid inhalation
Purity High purity grade
Packaging Available in various sizes
Shipping Restrictions May require special handling during shipping
Expiration Date Check expiration date on the label before use
Manufacturer Multiple manufacturers available
Product Code Unique code assigned by manufacturer
FAQ
What is the Acrylamide Solution (30%) and how is it used in molecular biology?

Acrylamide Solution (30%) is a commonly used reagent in molecular biology for various applications such as protein gel electrophoresis, DNA sequencing, and protein purification. It is a solution containing 30% acrylamide and is typically mixed with a crosslinking agent such as bisacrylamide in a ratio of 29:1 to form a polyacrylamide gel matrix.

What are the advantages of using the Acrylamide Solution (30%) in molecular biology experiments?

The Acrylamide Solution (30%) offers several advantages in molecular biology experiments. It provides excellent resolution for proteins and nucleic acids, has a high gel strength, and can be easily polymerized to form gels of various percentages. Additionally, it is compatible with a wide range of staining and detection methods commonly used in molecular biology research.

How should the Acrylamide Solution (30%) be stored and handled to ensure optimal performance?

The Acrylamide Solution (30%) should be stored at room temperature away from light and moisture to prevent degradation. It is important to handle the solution with care as acrylamide is a known neurotoxin and possible carcinogen. Always wear appropriate personal protective equipment such as gloves and lab coat when working with acrylamide solutions.

What precautions should be taken when working with the Acrylamide Solution (30%) in the laboratory?

When working with the Acrylamide Solution (30%), it is important to follow all safety guidelines and protocols established by your institution. Avoid inhalation, ingestion, or skin contact with the solution. In case of accidental exposure, immediately rinse the affected area with water and seek medical attention if necessary. Proper disposal of acrylamide waste is also essential to prevent environmental contamination.

Are there any alternative solutions to the Acrylamide Solution (30%) for molecular biology applications?

While the Acrylamide Solution (30%) is a widely used reagent in molecular biology, there are alternative solutions available for specific applications. Researchers can explore other gel matrices such as agarose for DNA electrophoresis or polyacrylamide alternatives with different crosslinking agents for protein separation. It is important to consider the specific requirements of your experiment and choose the most suitable reagent accordingly.
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