Acrylamide - Solution (40 %) - Mix 29 : 1 for molecular biology

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Product Name Acrylamide - Solution (40%) - Mix 29 : 1
Product Type Chemical reagent
Concentration 40%
Mix Ratio 29:1
Application Molecular biology
0 High purity
1 Ready-to-use solution
2 Used for polyacrylamide gel electrophoresis
3 Denaturing agent for protein separation
4 Compatible with SDS-PAGE
5 Stable at room temperature
6 Soluble in water
7 Store in a cool, dry place
8 Avoid exposure to light
9 Avoid contact with skin and eyes
FAQ
What is Acrylamide - Solution (40%) - Mix 29:1 used for in molecular biology?
Acrylamide - Solution (40%) - Mix 29:1 is commonly used for denaturing polyacrylamide gel electrophoresis in molecular biology. It is used for separating and analyzing nucleic acids and proteins based on their size.

How is Acrylamide - Solution (40%) - Mix 29:1 different from other acrylamide solutions?
Acrylamide - Solution (40%) - Mix 29:1 is specifically formulated with a 29:1 ratio of acrylamide to bis-acrylamide, which provides optimal resolution for DNA and RNA fragments of different sizes. This mix offers a balance between resolving power and gel longevity.

What are the advantages of using Acrylamide - Solution (40%) - Mix 29:1 for gel electrophoresis?
Acrylamide - Solution (40%) - Mix 29:1 offers high-resolution separation of nucleic acids and proteins due to its optimized ratio of acrylamide to bis-acrylamide. It provides sharp banding patterns, accurate sizing, and reproducible results, making it ideal for various molecular biology applications.

How should Acrylamide - Solution (40%) - Mix 29:1 be stored and handled?
Acrylamide - Solution (40%) - Mix 29:1 should be stored in a cool, dark place away from direct sunlight. Before use, it should be mixed thoroughly to ensure a homogeneous solution. Care should be taken when handling acrylamide solutions, as they are toxic and can cause skin and respiratory irritation.

What are some tips for optimizing gel electrophoresis using Acrylamide - Solution (40%) - Mix 29:1?
To optimize gel electrophoresis using Acrylamide - Solution (40%) - Mix 29:1, ensure that the gel is prepared accurately with the correct acrylamide concentration and buffer conditions. Use appropriate loading dyes and DNA markers for accurate sizing of DNA fragments. Running the gel at the appropriate voltage and for the right amount of time is crucial for achieving high-resolution results.
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