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| Name | Ammonium Sulfate |
| Application | Molecular Biology |
| Formula | (NH4)2SO4 |
| Molecular Weight | 132.14 g/mol |
| Appearance | White crystalline solid |
| Solubility | Soluble in water |
| Purity | ≥ 99.0% |
| Storage Condition | Store at room temperature |
| Usage | Precipitation of nucleic acids and proteins |
| PH | 5.5 - 7.0 |
| Cas Number | 7783-20-2 |
| Source | Synthetic |
| Grade | Molecular biology grade |
| Shelf Life | 2 years |
| Packaging | Bottles or bags |
| Manufacturer | Various manufacturers |
FAQ
What is Ammonium Sulfate and how is it used in molecular biology?
Ammonium Sulfate is an inorganic salt that is commonly used in molecular biology experiments for protein purification, particularly for precipitation of proteins. It is also used for crystallography applications and as a reagent for DNA and RNA extraction.
What are the advantages of using Ammonium Sulfate for molecular biology applications?
Ammonium Sulfate provides several advantages for molecular biology experiments. It is a highly soluble salt that is easy to handle and use. It can effectively precipitate proteins based on their solubility in different concentration ranges of Ammonium Sulfate. Additionally, it is cost-effective and widely available, making it a popular choice for protein purification techniques.
How is Ammonium Sulfate typically used for protein precipitation in molecular biology research?
In molecular biology research, Ammonium Sulfate is often used to selectively precipitate proteins from a solution based on their solubility at different salt concentrations. By gradually adding Ammonium Sulfate to a protein solution, researchers can manipulate the salt concentration to cause proteins to precipitate out of solution. The precipitated proteins can then be collected by centrifugation and further purified for downstream applications.
What are some best practices for using Ammonium Sulfate in molecular biology experiments?
When using Ammonium Sulfate for protein precipitation in molecular biology experiments, it is important to carefully control the salt concentration and pH of the solution. Researchers should also consider the specific solubility properties of the proteins they are working with to optimize the precipitation conditions. Additionally, proper safety precautions should be taken when handling Ammonium Sulfate, as it can be harmful if ingested or inhaled.
Are there any alternative methods for protein precipitation that can be used instead of Ammonium Sulfate in molecular biology research?
While Ammonium Sulfate is a commonly used reagent for protein precipitation in molecular biology research, there are other methods that can be used as alternatives. For example, ethanol or polyethylene glycol can also be used for protein precipitation, depending on the specific requirements of the experiment. Researchers may choose to explore different methods based on the nature of the proteins being studied and the downstream applications of the purified proteins.
Ammonium Sulfate is an inorganic salt that is commonly used in molecular biology experiments for protein purification, particularly for precipitation of proteins. It is also used for crystallography applications and as a reagent for DNA and RNA extraction.
What are the advantages of using Ammonium Sulfate for molecular biology applications?
Ammonium Sulfate provides several advantages for molecular biology experiments. It is a highly soluble salt that is easy to handle and use. It can effectively precipitate proteins based on their solubility in different concentration ranges of Ammonium Sulfate. Additionally, it is cost-effective and widely available, making it a popular choice for protein purification techniques.
How is Ammonium Sulfate typically used for protein precipitation in molecular biology research?
In molecular biology research, Ammonium Sulfate is often used to selectively precipitate proteins from a solution based on their solubility at different salt concentrations. By gradually adding Ammonium Sulfate to a protein solution, researchers can manipulate the salt concentration to cause proteins to precipitate out of solution. The precipitated proteins can then be collected by centrifugation and further purified for downstream applications.
What are some best practices for using Ammonium Sulfate in molecular biology experiments?
When using Ammonium Sulfate for protein precipitation in molecular biology experiments, it is important to carefully control the salt concentration and pH of the solution. Researchers should also consider the specific solubility properties of the proteins they are working with to optimize the precipitation conditions. Additionally, proper safety precautions should be taken when handling Ammonium Sulfate, as it can be harmful if ingested or inhaled.
Are there any alternative methods for protein precipitation that can be used instead of Ammonium Sulfate in molecular biology research?
While Ammonium Sulfate is a commonly used reagent for protein precipitation in molecular biology research, there are other methods that can be used as alternatives. For example, ethanol or polyethylene glycol can also be used for protein precipitation, depending on the specific requirements of the experiment. Researchers may choose to explore different methods based on the nature of the proteins being studied and the downstream applications of the purified proteins.
