Bisacrylamide for molecular biology

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Product Name Bisacrylamide
Product Type Chemical
Used For Molecular Biology
Appearance White powder
Molecular Weight 154.17 g/mol
Solubility Soluble in water
Storage Conditions Store at room temperature
Melting Point 163-165°C
Boiling Point 312-313°C
Purity ≥ 99%
Cas Number 110-26-9
FAQ
What is bisacrylamide and how is it used in molecular biology?

Bisacrylamide is a chemical compound commonly used in molecular biology as a crosslinking agent for polyacrylamide gels. It is often used in conjunction with acrylamide to create gels for electrophoresis, which allows for the separation of macromolecules based on their size and charge.

Why is bisacrylamide essential for molecular biology research?

Bisacrylamide plays a crucial role in molecular biology research by enabling scientists to separate and analyze DNA, RNA, and proteins. It is an essential component of polyacrylamide gels used in techniques such as gel electrophoresis, western blotting, and DNA sequencing.

How should bisacrylamide be handled and stored in the laboratory?

Bisacrylamide should be handled with care in the laboratory, as it is a potent neurotoxin and skin irritant. It is important to wear appropriate personal protective equipment, such as gloves and goggles, when working with bisacrylamide. Additionally, bisacrylamide should be stored in a cool, dry place away from direct sunlight and incompatible substances.

What are the benefits of using bisacrylamide in molecular biology applications?

The use of bisacrylamide in molecular biology applications offers several benefits, including high resolution and sharp banding patterns in polyacrylamide gels. Bisacrylamide is also versatile and can be used in a variety of techniques to study nucleic acids and proteins.

Are there any safety considerations when working with bisacrylamide in the laboratory?

Yes, there are several safety considerations to keep in mind when working with bisacrylamide in the laboratory. It is important to minimize exposure to bisacrylamide by using appropriate safety equipment and following good laboratory practices. In case of skin contact, it is crucial to wash the affected area thoroughly with soap and water.
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