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| Name | D(-)-Sorbitol |
| Product Type | Molecular Biology Product |
| Chemical Formula | C6H14O6 |
| Molecular Weight | 182.17 g/mol |
| Purity | ≥98% |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| Storage Conditions | Store at room temperature |
| Usage | Commonly used as a sugar substitute in cell culture media |
| Applications | Used as a cryoprotectant and osmoprotectant |
| Synonyms | Sorbitol, D-Glucitol |
| Cas Number | 50-70-4 |
| Manufacturer | Multiple manufacturers |
| Shelf Life | 2 years |
| Handling Precautions | Avoid inhalation, ingestion, and skin contact |
FAQ
What is D(-) Sorbitol and how is it used in molecular biology applications?
D(-) Sorbitol is a sugar alcohol that is commonly used in molecular biology as a cryoprotectant for cells and tissues. It helps to prevent damage to cells during the process of freezing and thawing, making it a valuable tool for preserving biological samples.
How does D(-) Sorbitol work as a cryoprotectant?
D(-) Sorbitol works by replacing water in cells and tissues, which helps to prevent ice crystal formation during freezing. This minimizes damage to the cellular structure and ensures that cells remain viable after thawing.
What are the benefits of using D(-) Sorbitol as a cryoprotectant in molecular biology?
Using D(-) Sorbitol as a cryoprotectant offers several benefits, including improved cell viability, increased sample integrity, and enhanced post-thaw recovery. It is also non-toxic to cells, making it a safe and effective choice for preserving biological samples.
How should D(-) Sorbitol be stored and handled for optimal results?
D(-) Sorbitol should be stored in a cool, dry place away from light and moisture. It is important to handle the product with care to prevent contamination and ensure its effectiveness as a cryoprotectant. Always follow the manufacturer's instructions for proper storage and handling.
Are there any potential drawbacks or limitations to using D(-) Sorbitol as a cryoprotectant?
While D(-) Sorbitol is generally considered safe and effective for use in molecular biology applications, some researchers have reported inconsistencies in cell viability and recovery rates when using this cryoprotectant. It is important to conduct thorough testing and optimization experiments to determine the optimal concentration and conditions for using D(-) Sorbitol in your specific application.
D(-) Sorbitol is a sugar alcohol that is commonly used in molecular biology as a cryoprotectant for cells and tissues. It helps to prevent damage to cells during the process of freezing and thawing, making it a valuable tool for preserving biological samples.
How does D(-) Sorbitol work as a cryoprotectant?
D(-) Sorbitol works by replacing water in cells and tissues, which helps to prevent ice crystal formation during freezing. This minimizes damage to the cellular structure and ensures that cells remain viable after thawing.
What are the benefits of using D(-) Sorbitol as a cryoprotectant in molecular biology?
Using D(-) Sorbitol as a cryoprotectant offers several benefits, including improved cell viability, increased sample integrity, and enhanced post-thaw recovery. It is also non-toxic to cells, making it a safe and effective choice for preserving biological samples.
How should D(-) Sorbitol be stored and handled for optimal results?
D(-) Sorbitol should be stored in a cool, dry place away from light and moisture. It is important to handle the product with care to prevent contamination and ensure its effectiveness as a cryoprotectant. Always follow the manufacturer's instructions for proper storage and handling.
Are there any potential drawbacks or limitations to using D(-) Sorbitol as a cryoprotectant?
While D(-) Sorbitol is generally considered safe and effective for use in molecular biology applications, some researchers have reported inconsistencies in cell viability and recovery rates when using this cryoprotectant. It is important to conduct thorough testing and optimization experiments to determine the optimal concentration and conditions for using D(-) Sorbitol in your specific application.
