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Name | EDTA - Solution pH 8.0 (0.5 M) |
Type | Molecular biology product |
Concentration | 0.5 M |
PH | 8 |
Ingredients | EDTA |
Form | Liquid |
Storage | Store at room temperature |
Stability | Stable for at least 1 year |
Usage | Used for chelating divalent metal ions in molecular biology applications |
Applications | DNA and RNA isolation, protein purification, cell culture |
Solubility | Soluble in water |
Color | Colorless |
Odor | Odorless |
Purity | High purity |
Buffer | Tris buffer |
Shelf Life | 2 years |
Size | 500 mL |
Manufacturer | Various manufacturers |
Safety Information | Harmful if swallowed, avoid contact with skin and eyes, wear appropriate protective clothing |
FAQ
What is EDTA Solution pH 8.0?
EDTA Solution pH 8.0 is a buffer solution containing EDTA (Ethylenediaminetetraacetic acid) at a concentration of 0.5M and adjusted to a pH of 8.0. This solution is commonly used in molecular biology applications for chelating metal ions, immobilizing metal ions, and preventing degradation of nucleic acids and proteins.
How can I use EDTA Solution pH 8.0 in my molecular biology experiments?
EDTA Solution pH 8.0 can be used in a variety of molecular biology applications, such as DNA and RNA isolation, PCR, gel electrophoresis, and protein purification. It helps to stabilize nucleic acids and proteins by chelating metal ions that could catalyze degradation reactions. Additionally, EDTA can be used to inhibit nucleases and proteases that may be present in biological samples.
What are the advantages of using EDTA Solution pH 8.0 in my experiments?
One of the main advantages of using EDTA Solution pH 8.0 is its ability to chelate metal ions, which helps to prevent enzyme activities and stabilize biological molecules. This can improve the overall quality and integrity of your nucleic acids and proteins, leading to more reliable and reproducible results in your experiments. Additionally, the pH of 8.0 is optimal for many molecular biology applications, ensuring that your samples remain stable and free from contamination.
Are there any considerations I should keep in mind when using EDTA Solution pH 8.0?
While EDTA Solution pH 8.0 is a versatile tool for molecular biology research, there are a few key considerations to keep in mind. First, it is important to handle EDTA with care, as it can be irritating to the skin and eyes. Additionally, EDTA can inhibit the activity of certain enzymes, so it is important to consider the specific requirements of your experiment when using this solution. Finally, be sure to store EDTA Solution pH 8.0 properly according to the manufacturer's recommendations to ensure its stability and effectiveness over time.
Can I customize the concentration or pH of EDTA Solution for my specific experimental needs?
If you require a different concentration or pH of EDTA Solution for your experiments, it is possible to customize the solution to meet your specific needs. Many suppliers offer custom solutions tailored to the requirements of individual researchers, so you can work with your supplier to create a solution that is optimized for your particular application.
EDTA Solution pH 8.0 is a buffer solution containing EDTA (Ethylenediaminetetraacetic acid) at a concentration of 0.5M and adjusted to a pH of 8.0. This solution is commonly used in molecular biology applications for chelating metal ions, immobilizing metal ions, and preventing degradation of nucleic acids and proteins.
How can I use EDTA Solution pH 8.0 in my molecular biology experiments?
EDTA Solution pH 8.0 can be used in a variety of molecular biology applications, such as DNA and RNA isolation, PCR, gel electrophoresis, and protein purification. It helps to stabilize nucleic acids and proteins by chelating metal ions that could catalyze degradation reactions. Additionally, EDTA can be used to inhibit nucleases and proteases that may be present in biological samples.
What are the advantages of using EDTA Solution pH 8.0 in my experiments?
One of the main advantages of using EDTA Solution pH 8.0 is its ability to chelate metal ions, which helps to prevent enzyme activities and stabilize biological molecules. This can improve the overall quality and integrity of your nucleic acids and proteins, leading to more reliable and reproducible results in your experiments. Additionally, the pH of 8.0 is optimal for many molecular biology applications, ensuring that your samples remain stable and free from contamination.
Are there any considerations I should keep in mind when using EDTA Solution pH 8.0?
While EDTA Solution pH 8.0 is a versatile tool for molecular biology research, there are a few key considerations to keep in mind. First, it is important to handle EDTA with care, as it can be irritating to the skin and eyes. Additionally, EDTA can inhibit the activity of certain enzymes, so it is important to consider the specific requirements of your experiment when using this solution. Finally, be sure to store EDTA Solution pH 8.0 properly according to the manufacturer's recommendations to ensure its stability and effectiveness over time.
Can I customize the concentration or pH of EDTA Solution for my specific experimental needs?
If you require a different concentration or pH of EDTA Solution for your experiments, it is possible to customize the solution to meet your specific needs. Many suppliers offer custom solutions tailored to the requirements of individual researchers, so you can work with your supplier to create a solution that is optimized for your particular application.