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| Name | EGTA for Molecular Biology |
| Size | 100g |
| Concentration | 0.5M |
| Purity | ≥99% |
| Storage Condition | Room temperature |
| Solubility | Soluble in water |
| PH | 6.5 - 7.5 |
| Usage | Chelator for divalent cations |
| Applications | Buffering agent, metal chelator |
| Appearance | White crystalline powder |
| Melting Point | 240-242°C |
| Boiling Point | Not available |
FAQ
What is EGTA and how is it used in molecular biology?
EGTA, or ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid, is a chelating agent commonly used in molecular biology to bind divalent metal ions, particularly calcium. It is often used to chelate calcium ions in biochemical and cell biological experiments.
Why is EGTA important in molecular biology research?
EGTA is important in molecular biology research because it helps to maintain calcium-free conditions in experiments where the presence of calcium ions could interfere with the results. By chelating calcium ions, EGTA can prevent unwanted reactions that could affect the outcome of an experiment.
How is EGTA typically used in molecular biology experiments?
EGTA is typically added to experimental solutions at a specific concentration to maintain calcium-free conditions. It is often used in cell culture experiments, enzyme assays, and other biochemical assays where the presence of calcium needs to be controlled. Depending on the specific experiment, EGTA may be added directly to the experimental solution or included in lysis buffers to prevent calcium-dependent enzyme activation.
Are there any limitations or considerations when using EGTA in molecular biology experiments?
While EGTA is a commonly used chelating agent in molecular biology, there are some limitations and considerations to keep in mind when using it. For example, EGTA may not be effective at chelating other divalent metal ions, such as magnesium, depending on the experimental conditions. Additionally, the pH of the solution can affect the ability of EGTA to chelate metal ions, so it is important to optimize the pH to ensure proper chelation.
What are the advantages of using EGTA in molecular biology research compared to other chelating agents?
EGTA is preferred in many molecular biology experiments due to its specificity for calcium ions and its relatively high affinity for binding calcium. Compared to other chelating agents, such as EDTA, EGTA has a higher selectivity for calcium over other divalent metal ions, making it a valuable tool for researchers studying calcium-dependent processes in cells. Additionally, EGTA is water-soluble and can be easily added to experimental solutions without causing precipitation or interfering with the experimental system.
EGTA, or ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid, is a chelating agent commonly used in molecular biology to bind divalent metal ions, particularly calcium. It is often used to chelate calcium ions in biochemical and cell biological experiments.
Why is EGTA important in molecular biology research?
EGTA is important in molecular biology research because it helps to maintain calcium-free conditions in experiments where the presence of calcium ions could interfere with the results. By chelating calcium ions, EGTA can prevent unwanted reactions that could affect the outcome of an experiment.
How is EGTA typically used in molecular biology experiments?
EGTA is typically added to experimental solutions at a specific concentration to maintain calcium-free conditions. It is often used in cell culture experiments, enzyme assays, and other biochemical assays where the presence of calcium needs to be controlled. Depending on the specific experiment, EGTA may be added directly to the experimental solution or included in lysis buffers to prevent calcium-dependent enzyme activation.
Are there any limitations or considerations when using EGTA in molecular biology experiments?
While EGTA is a commonly used chelating agent in molecular biology, there are some limitations and considerations to keep in mind when using it. For example, EGTA may not be effective at chelating other divalent metal ions, such as magnesium, depending on the experimental conditions. Additionally, the pH of the solution can affect the ability of EGTA to chelate metal ions, so it is important to optimize the pH to ensure proper chelation.
What are the advantages of using EGTA in molecular biology research compared to other chelating agents?
EGTA is preferred in many molecular biology experiments due to its specificity for calcium ions and its relatively high affinity for binding calcium. Compared to other chelating agents, such as EDTA, EGTA has a higher selectivity for calcium over other divalent metal ions, making it a valuable tool for researchers studying calcium-dependent processes in cells. Additionally, EGTA is water-soluble and can be easily added to experimental solutions without causing precipitation or interfering with the experimental system.
