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| Product Name | Formamide deionized for molecular biology |
| Category | Chemical reagent |
| Grade | Molecular biology grade |
| Purity | Deionized |
| Appearance | Colorless liquid |
| Odor | Slight ammonia-like odor |
| Solubility | Miscible in water |
| Storage | Store at room temperature |
| Usage | Commonly used in molecular biology applications |
| Stability | Stable under recommended storage conditions |
| CAS Number | 75-12-7 |
| Molecular Formula | CH3NO |
| Molar Mass | 45.04 g/mol |
FAQ
What is Formamide deionized and why is it used in molecular biology?
Formamide deionized is a reagent commonly used in molecular biology for a variety of applications. It is a denaturing agent that helps to break down hydrogen bonds, which are crucial for the stability of nucleic acids like DNA and RNA. By disrupting these hydrogen bonds, formamide deionized helps to separate double-stranded nucleic acids into single strands, making them more accessible for further analysis and manipulation.
How is Formamide deionized different from regular Formamide?
Formamide deionized is essentially the same as regular formamide, but with one key difference - it has been treated to remove any trace of ions or salts that could potentially interfere with molecular biology experiments. This deionization process ensures that the formamide is of the highest purity, making it ideal for applications where even tiny contaminants could cause significant issues.
What are some common uses of Formamide deionized in molecular biology?
Formamide deionized is commonly used in molecular biology for a variety of applications. Some of the most common uses include: - Denaturing nucleic acids for gel electrophoresis - Hybridization studies, such as Southern and Northern blotting - RNA sequencing and reverse transcription polymerase chain reaction (RT-PCR) - Resuspending DNA pellets for storage or further analysis - Adjusting the Tm (melting temperature) of DNA or RNA hybridization reactions.
How should Formamide deionized be stored and handled to ensure its stability and efficacy?
Formamide deionized should be stored at room temperature or below, away from direct sunlight and heat sources. It is important to keep the container tightly sealed when not in use to prevent contamination and evaporation. Before use, the formamide should be equilibrated to room temperature to minimize any condensation that may have formed in the bottle. When handling formamide deionized, it is important to wear appropriate personal protective equipment, such as gloves and safety goggles, to prevent skin and eye contact. Additionally, formamide deionized should be used in a well-ventilated area to minimize exposure to fumes.
Are there any safety considerations or precautions to keep in mind when working with Formamide deionized?
Formamide deionized is a potentially hazardous chemical and should be handled with care. It can be irritating to the skin, eyes, and respiratory tract, so it is important to avoid direct contact and inhalation of fumes. If contact does occur, wash the affected area with plenty of water and seek medical attention if necessary. In case of eye contact, rinse with water for at least 15 minutes and seek medical advice. It is also important to dispose of formamide deionized properly according to local regulations for hazardous waste.
Formamide deionized is a reagent commonly used in molecular biology for a variety of applications. It is a denaturing agent that helps to break down hydrogen bonds, which are crucial for the stability of nucleic acids like DNA and RNA. By disrupting these hydrogen bonds, formamide deionized helps to separate double-stranded nucleic acids into single strands, making them more accessible for further analysis and manipulation.
How is Formamide deionized different from regular Formamide?
Formamide deionized is essentially the same as regular formamide, but with one key difference - it has been treated to remove any trace of ions or salts that could potentially interfere with molecular biology experiments. This deionization process ensures that the formamide is of the highest purity, making it ideal for applications where even tiny contaminants could cause significant issues.
What are some common uses of Formamide deionized in molecular biology?
Formamide deionized is commonly used in molecular biology for a variety of applications. Some of the most common uses include: - Denaturing nucleic acids for gel electrophoresis - Hybridization studies, such as Southern and Northern blotting - RNA sequencing and reverse transcription polymerase chain reaction (RT-PCR) - Resuspending DNA pellets for storage or further analysis - Adjusting the Tm (melting temperature) of DNA or RNA hybridization reactions.
How should Formamide deionized be stored and handled to ensure its stability and efficacy?
Formamide deionized should be stored at room temperature or below, away from direct sunlight and heat sources. It is important to keep the container tightly sealed when not in use to prevent contamination and evaporation. Before use, the formamide should be equilibrated to room temperature to minimize any condensation that may have formed in the bottle. When handling formamide deionized, it is important to wear appropriate personal protective equipment, such as gloves and safety goggles, to prevent skin and eye contact. Additionally, formamide deionized should be used in a well-ventilated area to minimize exposure to fumes.
Are there any safety considerations or precautions to keep in mind when working with Formamide deionized?
Formamide deionized is a potentially hazardous chemical and should be handled with care. It can be irritating to the skin, eyes, and respiratory tract, so it is important to avoid direct contact and inhalation of fumes. If contact does occur, wash the affected area with plenty of water and seek medical attention if necessary. In case of eye contact, rinse with water for at least 15 minutes and seek medical advice. It is also important to dispose of formamide deionized properly according to local regulations for hazardous waste.
