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| Product Name | Glycine for Molecular Biology |
| CAS Number | 56-40-6 |
| Molecular Formula | C2H5NO2 |
| Molecular Weight | 75.07 g/mol |
| Purity | ≥ 98% |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| Storage Conditions | Store at room temperature |
| Usage | Commonly used as a protein building block in molecular biology |
| Shelf Life | 2 years |
| Synonyms | Aminoacetic acid, Glycocoll |
| Handling Precautions | Avoid inhalation or ingestion, wear protective gloves and clothing |
FAQ
What is glycine and how is it used in molecular biology research?
Glycine is a non-essential amino acid that plays a critical role in various biological processes. In molecular biology research, it is commonly used as a protein stabilizer, pH regulator, and a reagent in electrophoresis.
How does glycine act as a protein stabilizer?
Glycine is often used as a protein stabilizer due to its ability to prevent protein aggregation and denaturation. By acting as a buffer, it helps maintain the proper pH levels that are essential for preserving the structure and function of proteins.
What are some applications of glycine in molecular biology?
Glycine is widely used in molecular biology for a variety of applications, including protein purification, western blotting, and SDS-PAGE gel electrophoresis. It is also used in cell culture media to support cell growth and viability.
How is glycine used in western blotting?
In western blotting, glycine is commonly used as a blocking agent to prevent nonspecific binding of antibodies to the membrane. By incubating the membrane with a glycine solution, researchers can effectively block unwanted interactions and improve the specificity of their results.
Can glycine be used in conjunction with other reagents in molecular biology experiments?
Yes, glycine is often used in combination with other reagents such as SDS, Tris, and acrylamide for various molecular biology experiments. Its compatibility with a wide range of reagents makes it a versatile tool for researchers in the field.
Glycine is a non-essential amino acid that plays a critical role in various biological processes. In molecular biology research, it is commonly used as a protein stabilizer, pH regulator, and a reagent in electrophoresis.
How does glycine act as a protein stabilizer?
Glycine is often used as a protein stabilizer due to its ability to prevent protein aggregation and denaturation. By acting as a buffer, it helps maintain the proper pH levels that are essential for preserving the structure and function of proteins.
What are some applications of glycine in molecular biology?
Glycine is widely used in molecular biology for a variety of applications, including protein purification, western blotting, and SDS-PAGE gel electrophoresis. It is also used in cell culture media to support cell growth and viability.
How is glycine used in western blotting?
In western blotting, glycine is commonly used as a blocking agent to prevent nonspecific binding of antibodies to the membrane. By incubating the membrane with a glycine solution, researchers can effectively block unwanted interactions and improve the specificity of their results.
Can glycine be used in conjunction with other reagents in molecular biology experiments?
Yes, glycine is often used in combination with other reagents such as SDS, Tris, and acrylamide for various molecular biology experiments. Its compatibility with a wide range of reagents makes it a versatile tool for researchers in the field.
